FE - Artigos de Revistas Internacionais com Arbitragem Científica

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    Microfluidic bioreactors for enzymatic synthesis in packed-bed reactors—Multi-step reactions and upscaling
    (Elsevier B.V., 2020-11-10) Brás, Eduardo J.S.; Domingues, Cristiana; Chu, Virginia; Fernandes, Pedro; Conde, João Pedro; HEI-LAB (FCT) - Digital Laboratories for Environments and Human Interactions
    Enzymatic synthesis of biochemical commodities is of upmost importance as it represents a greener alternative to traditional chemical synthesis and provides easier downstream processing strategies compared to fermentation-based processes. A microfluidic system used to optimize the enzymatic production of both levodopa (L-DOPA) and dopamine in both single-step and multistep-reaction sequences with yield of approximately 30 % for L-DOPA production and 70 % for dopamine production is presented. The system for L-DOPA production was then up-scaled (780-fold increase) to a milliliter scale system by maintaining similar mass transport properties resulting in the same yield, space-time yield and biocatalyst yield as its microscale counterpart. The results obtained for yield and biocatalyst yield (351.7 mgL-DOPA mg−1Tyr h−1) were similar to what is reported in the literature for similar systems, however the space-time yield (0.806 mgL-DOPA L−1 h−1) was smaller. This work demonstrates a microfluidic bioreactor that can be used for complex optimizations that can be performed rapidly while reducing the consumption of reagents by immobilizing the catalyst on a carrier which can then be used in a packed-bed reactor, thus extending the enzyme life span.