Obtenção de extractos de azeitona em conserva usando CO2 supercrítico e avaliação preliminar do seu conteúdo em polifenóis
Miniatura indisponível
Data
2014
Autores
Título da revista
ISSN da revista
Título do Volume
Editora
Edições Lusófonas
Resumo
A técnica de Extração Supercrítica (ESC) foi usada para obter extractos de uma amostra, que pretendia simular
o bagaço de azeitona, obtida de azeitonas em conserva. O objetivo era conseguir a extração de componentes
polifenólicos desta matriz que é considerada um resíduo perigoso proveniente da produção de azeite. O fluido
supercrítico usado foi o dióxido de carbono e os estudos de SFE foram realizados em duas fases: a primeira, a uma
pressão de 200 bar e 40 ° C e a segunda a 300 bar, 45º C e usando etanol como co-solvente. Em ambos os casos, a SFE foi realizada durante 3 horas. A primeira fase foi realizada para obter uma matriz mais limpa e a segunda etapa tinha como alvo os componentes de interesse. O rendimento total em massa obtido foi de 5,5 %. O estudo
preliminar, por HPLC, do perfil químico foi efectuado e o rastreio das amostras obtidas foi realizado, bem como o da água da conserva das azeitonas, e mostraram que os compostos polifenólicos estavam presentes principalmente
neste último caso.
The supercritical fluid extraction (SFE) technique was used to obtain extracts from a sample simulating an olive pomace, obtained from preserved olives. The objective was to achieve the extraction of polyphenolic components from this matrix which is considered a hazardous waste from the production of olive oil. The supercritical fluid used was carbon dioxide and the SFE studies were conducted in two stages, the first at a pressure of 200 bar and 40ºC and the second stage at 300 bar, 45 ºC and using ethanol as co-solvent. In both cases the SFE was performed for 3 hours. The first stage was carried out to obtain a cleaner matrix and the second step was aimed at the components of interest. The overall mass yield obtained was 5.5 %. Preliminary HPLC screening of the samples obtained and also of the water in which the olives were preserved showed that the polyphenol compounds were mostly in the latter.
The supercritical fluid extraction (SFE) technique was used to obtain extracts from a sample simulating an olive pomace, obtained from preserved olives. The objective was to achieve the extraction of polyphenolic components from this matrix which is considered a hazardous waste from the production of olive oil. The supercritical fluid used was carbon dioxide and the SFE studies were conducted in two stages, the first at a pressure of 200 bar and 40ºC and the second stage at 300 bar, 45 ºC and using ethanol as co-solvent. In both cases the SFE was performed for 3 hours. The first stage was carried out to obtain a cleaner matrix and the second step was aimed at the components of interest. The overall mass yield obtained was 5.5 %. Preliminary HPLC screening of the samples obtained and also of the water in which the olives were preserved showed that the polyphenol compounds were mostly in the latter.
Descrição
Palavras-chave
AZEITONA, OLIVE, CIÊNCIAS FARMACÊUTICAS, PHARMACEUTICAL SCIENCES, AZEITONA, OLIVE, SUBPRODUTOS, BY-PRODUCTS, ESTUDOS DE CASO, CASE STUDIES, POLIFENÓIS, POLYPHENOLS