A high-throughput screening method for general cytotoxicity part I Chemical toxicity
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Data
2005
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Edições Universitárias Lusófonas
Resumo
Foi desenvolvido um método destinado a fazer a triagem rápida e o escalonamento da toxicidade geral exercida por xenobióticos tendo como modelo o Saccharomyces cerevisiae. Para padronizar as condições de experimentação foi
estabelecida a relação entre a absorvência a 525 nm e o número de células em suspensão por mililitro de meio de cultura e calculadas uma curva padrão e respectiva equação definidora (Y=6,8219E-08X + 0,0327) Culturas de Saccharomyces
cerevisiae em meio completo para leveduras (YPD - 1% de glucose 2%, de peptona 0,5% e extracto de levedura 1%) foram expostas a diferentes concentrações de nicotina e a inibição do crescimento avaliada.
A high-throughput assay intended for screening and ranking the general toxicity of physical or chemical xenobiotics based on a Saccharomyces cerevisiae model was developed. In order to standardise the running conditions for the technique the relationship between the absorbance at 525 nm and the number of cells/ml suspended in YPD culture medium (YPD - yeast extract 1%, peptone 0.5% and glucose 2%) was determined and a standard curve, described by the equation Y=6.8219E-08X + 0.0327. Cultures of yeast in YPD medium were exposed to different concentrations of nicotine and the growth inhibition evaluated. We could demonstrate by covariance analysis the reproducibility of the results obtained and that method is valid and suitable for its aims.
A high-throughput assay intended for screening and ranking the general toxicity of physical or chemical xenobiotics based on a Saccharomyces cerevisiae model was developed. In order to standardise the running conditions for the technique the relationship between the absorbance at 525 nm and the number of cells/ml suspended in YPD culture medium (YPD - yeast extract 1%, peptone 0.5% and glucose 2%) was determined and a standard curve, described by the equation Y=6.8219E-08X + 0.0327. Cultures of yeast in YPD medium were exposed to different concentrations of nicotine and the growth inhibition evaluated. We could demonstrate by covariance analysis the reproducibility of the results obtained and that method is valid and suitable for its aims.
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Revista Lusófona de Ciências e Tecnologias da Saúde
Palavras-chave
MEDICINA, TOXICOLOGIA, TOXICIDADE, MEIOS DE DIAGNÓSTICO, MEDICINE, TOXICOLOGY, TOXICITY, DIAGNOSTIC PROCEDURES, XENOBIÓTICOS, XENOBIOTICS