Cold-induced skin perfusion adaptive responses : comparing ICE and PORH mechanisms In Vivo
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Data
2025
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Edições Universitárias Lusófonas
Resumo
O arrefecimento por exposição pelo gelo (AEG) é uma técnica interessante embora pouco explorada para examinar os mecanismos adaptativos da microcirculação periférica humana. Neste estudo, comparamos as alterações de perfusão in vivo avaliadas por fluxometria laser Doppler (LDF) envolvendo a hiperemia reactiva pósoclusão (HRPO) e o AEG. Onze mulheres saudáveis, com 29,3±7,3 anos de idade, foram selecionadas e submetidas sequencialmente aos procedimentos de HRPO e AEG num membro superior selecionado aleatoriamente. A perfusão sanguínea e a temperatura da pele foram medidas continuamente em ambas as mãos durante a linha de base (fase I), no desafio (fase II) e na recuperação (fase III). Os procedimentos respeitaram todos os princípios de investigação clínica e foram previamente submetidos ao comité de ética institucional. A HRPO e o AEG evocaram diminuições significativas na perfusão da pele na fase II, seguidas de reperfusão no membro intervencionado, embora com velocidades diferentes. Respostas significativas semelhantes foram obtidas nos membros contralaterais. Não foi possível encontrar correlação entre as variáveis, exceto entre os sinais de LDF e temperatura ao longo das fases. Os resultados sugerem que o ICE é melhor tolerado do que o POHR quando utilizado com o mesmo objetivo. São necessários mais estudos para confirmar se os reflexos provocados por estas abordagens são equivalentes e igualmente adequados para exploração em medicina vascular.
Ice cooling exposure (ICE) is an interesting, but little explored, technique used to examine human peripheral microcirculatory adaptive mechanisms. In this study, we compare in vivo perfusion changes assessed by laser Doppler flowmetry (LDF) during post-occlusive reactive hyperemia (POHR) and ICE procedures. Eleven healthy women, 29.3±7.3 years old, were selected and sequentially submitted to PORH and ICE procedures in one randomly selected upper limb. Blood perfusion and skin temperature were continuously measured in both hands during baseline (phase I) challenge (phase II) and recovery (phase III). Procedures respected all clinical research principles and were previously submitted to the institutional ethics committee. PORH and ICE evoked significant decreases in skin perfusion in phase II followed by reperfusion in the intervention limb, although with different velocities. Similar significant responses were obtained in the contralateral limbs. Correlation among variables could not be found, except between LDF and temperature signals across phases. Results suggest that ICE is better tolerated than POHR when used with the same objective. More studies are needed to confirm if reflexes elicited by these approaches are equivalent and equally suitable for application in vascular medicine.
Ice cooling exposure (ICE) is an interesting, but little explored, technique used to examine human peripheral microcirculatory adaptive mechanisms. In this study, we compare in vivo perfusion changes assessed by laser Doppler flowmetry (LDF) during post-occlusive reactive hyperemia (POHR) and ICE procedures. Eleven healthy women, 29.3±7.3 years old, were selected and sequentially submitted to PORH and ICE procedures in one randomly selected upper limb. Blood perfusion and skin temperature were continuously measured in both hands during baseline (phase I) challenge (phase II) and recovery (phase III). Procedures respected all clinical research principles and were previously submitted to the institutional ethics committee. PORH and ICE evoked significant decreases in skin perfusion in phase II followed by reperfusion in the intervention limb, although with different velocities. Similar significant responses were obtained in the contralateral limbs. Correlation among variables could not be found, except between LDF and temperature signals across phases. Results suggest that ICE is better tolerated than POHR when used with the same objective. More studies are needed to confirm if reflexes elicited by these approaches are equivalent and equally suitable for application in vascular medicine.
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Publisher Copyright: © 2025 ALIES. All rights reserved.
Palavras-chave
MEDICINE, REACTIVE HYPEREMIA, MICROCIRCULATION, ICE, LASER-DOPPLER FLOWMETRY, MEDICINA, HIPEREMIA REATIVA, MICROCIRCULAÇÃO, GELO, FLUXOMETRIA POR LASER-DOPPLER
Citação
Caetano, J, de la Villa Polo, P, Alves, J D & Rodrigues, L M 2025, 'Cold-induced skin perfusion adaptive responses : comparing ICE and PORH mechanisms In Vivo', Biomedical and Biopharmaceutical Research, vol. 22, no. 1, pp. 94-105. https://doi.org/10.19277/bbr.22.1.355