Catarino, José Carlos MotaViegas, InêsRodrigues, JoanaPinto, MartaFaísca, Pedro2018Catarino, J C M, Viegas, I, Rodrigues, J, Pinto, M & Faísca, P 2018, 'Quantifying hepatic fibrosis on murine models : how to obtain representative results in a less laborious way', Revista Lusófona de Ciência e Medicina Veterinária, vol. 9, no. 9, pp. 17-22. https://doi.org/10.60543/rlcmv.v9i0.66581646-8805http://hdl.handle.net/10437/9492Revista Lusófona de Ciência e Medicina VeterináriaIn biomedical research, quantification of histological images is often required. Many of the methods used are time-consuming, laborious, originate variable results and are difficult to replicate. This paper is aimed towards finding a more objective, reproducible and easy to perform method to obtain representative results in murine models of hepatic fibrosis using ImageJ software. To do so on a liver fibrosis model, the percentages of fibrotic lesion obtained in an entire section and in several different magnifications were compared. No statistically significant differences were found (p> 0.05), but the correlation was stronger between the results obtained in the entire section photograph and the two photographs at 40x (r = 0.963). Using ImageJ facilitated the definition of a methodology that originated representative results on a liver section and at the same time allowed objective measurement in a reproducible and less laborious way.In biomedical research, quantification of histological images is often required. Many of the methods used are time-consuming, laborious, originate variable results and are difficult to replicate. This paper is aimed towards finding a more objective, reproducible and easy to perform method to obtain representative results in murine models of hepatic fibrosis using ImageJ software. To do so on a liver fibrosis model, the percentages of fibrotic lesion obtained in an entire section and in several different magnifications were compared. No statistically significant differences were found (p> 0.05), but the correlation was stronger between the results obtained in the entire section photograph and the two photographs at 40x (r = 0.963).application/pdfengopenAccessIMAGEJFÍGADOMORFOMETRIAVETERINÁRIAMEDICINA VETERINÁRIAFIBROSEPATOLOGIA EXPERIMENTALVETERINARY MEDICINEFIBROSISLIVERMORPHOMETRYEXPERIMENTAL PATHOLOGYarticlehttps://doi.org/10.60543/rlcmv.v9i0.6658