Catarino, José Carlos MotaViegas, InêsRodrigues, JoanaPinto, MartaFaísca, Pedro2019-04-092019-04-092019http://hdl.handle.net/10437/9492Revista Lusófona de Ciência e Medicina VeterináriaIn biomedical research, quantification of histological images is often required. Many of the methods used are time-consuming, laborious, originate variable results and are difficult to replicate. This paper is aimed towards finding a more objective, reproducible and easy to perform method to obtain representative results in murine models of hepatic fibrosis using ImageJ software. To do so on a liver fibrosis model, the percentages of fibrotic lesion obtained in an entire section and in several different magnifications were compared. No statistically significant differences were found (p> 0.05), but the correlation was stronger between the results obtained in the entire section photograph and the two photographs at 40x (r = 0.963). Using ImageJ facilitated the definition of a methodology that originated representative results on a liver section and at the same time allowed objective measurement in a reproducible and less laborious way.application/pdfengopenAccessIMAGEJVETERINÁRIAMEDICINA VETERINÁRIAFIBROSEFÍGADOMORFOMETRIAVETERINARY MEDICINEFIBROSISLIVERMORPHOMETRYPATOLOGIA EXPERIMENTALEXPERIMENTAL PATHOLOGYarticle