Efeitos sobre a expressão de genes ligando transmembranar de linfócitos B após tratamento com extrato de Rosa
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2023
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Edições Universitárias Lusófonas
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Pretendemos avaliar o papel do extrato da rosa vermelha (Pierre de Ronsard) na expressão genética de CD20, CD30, CD40 e CCR5 em linfócitos B humanos. O extrato foi preparado na diluição de 0,0075% (v/v) e armazenado a -20°C até à sua utilização. O tratamento celular foi efectuado a 37°C. As células foram colocadas em placas de 6 poços a 1,5x106 células por poço e armazenadas a -80°C, extraindo o ARN total. A RTq-PCR foi efectuada de acordo com a Genecopoeia. Utilizámos o método do limiar do ciclo (ΔΔCt) para a análise dos dados. A quantificação comparativa do método Ct (2^-ΔCt ) e a alteração fold para CD20, CD30, CD40 e CCR5 foram - 5,65E+01, 4,80E-01, N/A, 2,47E- 01; e 0,954,0,377, N/A e 0,577, respetivamente. A quantidade de ARN total extraída de cerca de 4,5x10⁶ células foi baixa e não permitiu medir o perfil de ARN. Com excepção do CD 40, todos os outros genes foram expressos e bem medidos em ambas as amostras de células B por qRT-PCR. Em conclusão, o tratamento com extrato de rosa diminuiu a expressão dos genes CD30 e CCR5 nos linfócitos B. São necessários mais estudos para aprofundar estes efeitos e o seu potencial. Palavras-chave: Extrato de rosa, linfócitos B, CD30, CCR5
The goal was to evaluate the role of red rose extract (Pierre de Ronsard) on the human B lymphocytes gene CD20, CD30, CD40, and CCR5 expression. Red rose extract was prepared at the dilution of 0.0075% (v/v) and stored until use at -20°C. Cell treatment was performed at 37°C on human B cells. Cells were plated in 6 well plates at 1.5x106 cells per well and stored at -80°C and total RNA extracted. RTq-PCR was performed according to Genecopoeia. The cycle threshold method (ΔΔCt) was used for data analysis. The comparative Ct method quantification (2^-ΔCt ) and fold change for CD20, CD30, CD40 and CCR5 were - 5.65E+01, 4.80E-01, N/A, 2.47E-01; and 0.954,0.377, N/A and 0.577, respectively. The amount of total RNA extracted from about 4.5x10⁶ cells was low and did not allow us to measure the RNA profile. With the exception of CD40, all other genes were expressed and well-measured in both B cell samples by qRT-PCR. The expression of CD30 and CCR5 genes were decreased in B lymphocytes in vitro with the rose extract treatment. More studies are needed to further study these effects and potential. Keywords: Rose extract, B lymphocytes, CD30, CCR5
The goal was to evaluate the role of red rose extract (Pierre de Ronsard) on the human B lymphocytes gene CD20, CD30, CD40, and CCR5 expression. Red rose extract was prepared at the dilution of 0.0075% (v/v) and stored until use at -20°C. Cell treatment was performed at 37°C on human B cells. Cells were plated in 6 well plates at 1.5x106 cells per well and stored at -80°C and total RNA extracted. RTq-PCR was performed according to Genecopoeia. The cycle threshold method (ΔΔCt) was used for data analysis. The comparative Ct method quantification (2^-ΔCt ) and fold change for CD20, CD30, CD40 and CCR5 were - 5.65E+01, 4.80E-01, N/A, 2.47E-01; and 0.954,0.377, N/A and 0.577, respectively. The amount of total RNA extracted from about 4.5x10⁶ cells was low and did not allow us to measure the RNA profile. With the exception of CD40, all other genes were expressed and well-measured in both B cell samples by qRT-PCR. The expression of CD30 and CCR5 genes were decreased in B lymphocytes in vitro with the rose extract treatment. More studies are needed to further study these effects and potential. Keywords: Rose extract, B lymphocytes, CD30, CCR5
Descrição
Biomedical and biopharmaceutical research : jornal de investigação biomédica e biofarmacêutica
Palavras-chave
CIÊNCIAS FARMACÊUTICAS, LINFÓCITOS, PHARMACEUTICAL SCIENCES, LYMPHOCYTES
Citação
Arokiaraj, M C & Menesson, E 2023, 'Efeitos sobre a expressão de genes ligando transmembranar de linfócitos B após tratamento com extrato de Rosa', Biomedical and biopharmaceutical research : jornal de investigação biomédica e biofarmacêutica.