Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes

Ver registo simples
dc.contributor.authorFachada, Vasco
dc.contributor.authorRahkila, Paavo
dc.contributor.authorFachada, Nuno
dc.contributor.authorTurpeinen, Tuomas
dc.contributor.authorKujala, Urho M.
dc.contributor.authorKainulainen, Heikki
dc.contributor.institutionCOPELABS - Cognitive and People-centric Computing
dc.date.issued2022-04
dc.description.abstractSkeletal muscle physiology remains of paramount importance in understanding insulin resistance. Due to its high lipid turnover rates, regulation of intramyocellular lipid droplets (LDs) is a key factor. Perilipin 5 (PLIN5) is one of the most critical agents in such regulation, being often referred as a protector against lipotoxicity and consequent skeletal muscle insulin resistance. We examined area fraction, size, subcellular localization and PLIN5 association of LDs in two fiber types of type 2 diabetic (T2D), obese (OB) and healthy (HC) individuals by means of fluorescence microscopy and image analysis. We found that T2D type II fibers have a significant sub-population of large and internalized LDs, uncoated by PLIN5. Based on this novel result, additional hypotheses for the pathophysiology of skeletal muscle insulin resistance are formulated, together with future research directions.en
dc.description.sponsorshipThis study was supported by the Academy of Finland (grant 132987 and 298875) and Fundaç˜ ao para a Ciˆ encia e a Tecnologia (FCT, Portugal) under grant UIDB/04111/2020 (COPELABS). The author Vasco Fachada acknowledges his FCT grant SFRH/BD/68308/2010
dc.formatapplication/pdf
dc.identifier.citationFachada, V, Rahkila, P, Fachada, N, Turpeinen, T, Kujala, U M & Kainulainen, H 2022, 'Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes', Acta Histochemica, vol. 124, no. 3, 151869. https://doi.org/10.1016/j.acthis.2022.151869
dc.identifier.doihttps://doi.org/10.1016/j.acthis.2022.151869
dc.identifier.issn0065-1281
dc.identifier.urlhttps://www.scopus.com/pages/publications/85125120283
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier GmbH
dc.relation.ispartofActa Histochemica
dc.rightsopenAccess
dc.subjectBIOLOGIA
dc.subjectLÍPIDOS
dc.subjectDIABETES TIPO 2
dc.subjectMÚSCULOS ESQUELÉTICOS
dc.subjectRESISTÊNCIA À INSULINA
dc.subjectBIOLOGY
dc.subjectLIPIDS
dc.subjectTYPE 2 DIABETES
dc.subjectSKELETAL MUSCLE
dc.subjectINSULIN RESISTANCE
dc.titleEnlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetesen
dc.typearticle

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
2022_lipiddroplets.pdf
Tamanho:
4.47 MB
Formato:
Adobe Portable Document Format
Descrição:
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

FE - Artigos de Revistas Internacionais com Arbitragem Científica