Avaliação preliminar da segurança do n-butanol do processo de extração de colágeno e do extrato de colágeno da pele de Oreochromis niloticus (tilápia) para uso dermocosmético
Miniatura indisponível
Ficheiros
Data
2021
Título da revista
ISSN da revista
Título do Volume
Editora
Edições Universitárias Lusófonas
Resumo
A obtenção de novos ingredientes cosméticos, principalmente de fontes sustentáveis, como excipientes ou mesmo compostos ativos, é notável para a indústria cosmética para fórmulas dermocosméticas inovadoras. Assim, é essencial estabelecer a segurança desses novos ingredientes para evitar eventos adversos, principalmente aqueles associados aos efeitos clastogênicos dos compostos químicos utilizados para extração de colágeno. Avaliamos soluções de um composto químico utilizado no processo de extração de colágeno da pele de tilápia (Oreochromis niloticus). Os efeitos citotóxicos e genotóxicos das soluções utilizadas no processo de extração de colágeno foram de 10,0; 1,0; 0,5; 0,1; 0,05; e 0,025% de n-butanol. Estas foram avaliadas pelo teste de Allium e pelo ensaio do cometa em glóbulos brancos periféricos. Ademais, foram investigadas a água residual da última lavagem na fase pré-tratamento e o extrato de colágeno de tilápia liofilizado a 0,5%. A ausência de atividade citotóxica e genotóxica foi demonstrada no extrato de colágeno, apesar do n-butanol ter demonstrado dano ao DNA, tanto nas células radiculares de Allium cepa quanto nos glóbulos brancos do sangue periférico humano. Por isso, observamos a necessidade de realização de testes de genotoxicidade para garantir a ausência de contaminantes no extrato de colágeno para fins dermocosméticos. Palavras-chave: Teste de Allium; extração de colágeno; ensaio de cometa; citotoxicidade; genotoxicidade; Oreochromis niloticus
Obtaining new cosmetic ingredients, mainly from sustainable sources, as novel excipiente or even active compounds is noteworthy for the cosmetic industry to enhance new innovative dermocosmetics. Thus, it is essential to establish the safety of these new ingredients to avoid adverse events, mainly those associated with clastogenic effects from the chemical compounds used for collagen extraction. In this study, we evaluated solutions of chemical compounds used in the collagen extraction process from tilapia skin (Oreochromis niloticus). The cytotoxic and genotoxic effects of the solutions used in the collagen extraction process were 10.0, 1.0, 0.5, and 0.1% n-butanol. Solutions were evaluated by the Allium test and the comet assay in peripheral white blood cells. The residual water from the final skin wash in the pre-treatment phase and the 0.5% lyophilized collagen extract were also investigated. The absence of cytotoxic and genotoxic activity was demonstrated in the collagen extract, despite the fact that n-butanol showed DNA damage, both in the root cells of Allium cepa and in the white blood cells of human peripheral blood. Therefore, we note the necessity to carry out genotoxicity tests to guarantee the absence of contaminants in the collagen extract for cosmetic purposes. Keywords: Allium test; collagen extraction; Comet assay; cytotoxicity; genotoxicity; Oreochromis niloticus
Obtaining new cosmetic ingredients, mainly from sustainable sources, as novel excipiente or even active compounds is noteworthy for the cosmetic industry to enhance new innovative dermocosmetics. Thus, it is essential to establish the safety of these new ingredients to avoid adverse events, mainly those associated with clastogenic effects from the chemical compounds used for collagen extraction. In this study, we evaluated solutions of chemical compounds used in the collagen extraction process from tilapia skin (Oreochromis niloticus). The cytotoxic and genotoxic effects of the solutions used in the collagen extraction process were 10.0, 1.0, 0.5, and 0.1% n-butanol. Solutions were evaluated by the Allium test and the comet assay in peripheral white blood cells. The residual water from the final skin wash in the pre-treatment phase and the 0.5% lyophilized collagen extract were also investigated. The absence of cytotoxic and genotoxic activity was demonstrated in the collagen extract, despite the fact that n-butanol showed DNA damage, both in the root cells of Allium cepa and in the white blood cells of human peripheral blood. Therefore, we note the necessity to carry out genotoxicity tests to guarantee the absence of contaminants in the collagen extract for cosmetic purposes. Keywords: Allium test; collagen extraction; Comet assay; cytotoxicity; genotoxicity; Oreochromis niloticus
Descrição
Biomedical and biopharmaceutical research : jornal de investigação biomédica e biofarmacêutica
Palavras-chave
CIÊNCIAS FARMACÊUTICAS, PRODUTOS COSMÉTICOS, CITOTOXICIDADE, GENOTOXICIDADE, PEIXES, PHARMACEUTICAL SCIENCES, COSMETICS, CYTOTOXICITY, GENOTOXICITY, FISHES