Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis

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dc.contributor.authorTůma, S.
dc.contributor.authorIzaguirre, J. K.
dc.contributor.authorBondar, M.
dc.contributor.authorMarques, M. M.
dc.contributor.authorFernandes, P.
dc.contributor.authorda Fonseca, M. M.R.
dc.contributor.authorCesário, M. T.
dc.contributor.institutionFE - Faculty of Engineering
dc.date.issued2020-09
dc.description.abstractAgar extraction from Gelidium and Gracilaria red seaweed species produces hundred thousand ton of carbohydrate-rich residues annually. Gelidium sesquipedale waste biomass obtained after agar extraction, still contained 44.2 % w/w total carbohydrates (dry-weight basis). These residues were biologically up-graded to poly-3-hydroxybutyrate (P3HB) after saccharification of their carbohydrate fraction to simple sugars. A combined hydrolysis treatment using sulfamic acid followed by enzymatic hydrolysis with cellulases produced a glucose-rich hydrolysate with a negligible content of inhibitors. With this treatment a sugar yield of circa 30 % (g glucose/g biomass) was attained. The algal hydrolysates were assessed as carbon source for the production of P3HB by the halotolerant bacteria Halomonas boliviensis. A cell concentration of 8.3 g L−1 containing 41 % (w/w) of polymer and a yield (YP/S) of 0.16 gpolymer/gglucose were attained in shake flask assays. In this work, cellulose-rich seaweed waste was shown to be an upgradable, sustainable source of carbohydrates.en
dc.description.sponsorshipThis work has received funding from FCT-Fundação para a Ciência e Tecnologia under the project (PTDC/BII-BIO/29242/2017). Funding received by iBB-Institute for Bioengineering and Biosciences from FCT (grant UIDB/04565/2020) and from Programa Operacional Regional de Lisboa 2020 (Project N. 007317) is also acknowledged. Štepan Tuma acknowledges support from the ERASMUS Plus program (EU) and Jon Kepa Izaguirre acknowledges the Basque Government for the pre-doctoral fellowship that supported his staying at iBB-IST. The authors would like to acknowledge IBERAGAR S.A., Portugal for kindly supplying the Gelidium residues.
dc.formatapplication/pdf
dc.identifier.citationTůma, S, Izaguirre, J K, Bondar, M, Marques, M M, Fernandes, P, da Fonseca, M M R & Cesário, M T 2020, 'Upgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensis', Biotechnology Reports, vol. 27, e00491. https://doi.org/10.1016/j.btre.2020.e00491
dc.identifier.doihttps://doi.org/10.1016/j.btre.2020.e00491
dc.identifier.issn2215-017X
dc.identifier.urlhttps://www.scopus.com/pages/publications/85086596040
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier B.V.
dc.relation.ispartofBiotechnology Reports
dc.rightsopenAccess
dc.subjectBIOTECNOLOGIA
dc.subjectGELIDIUM SESQUIPEDALE
dc.subjectHALOMONAS BOLIVIENSIS
dc.subjectRESÍDUOS DE ALGAS MARINHAS
dc.subjectPOLI-3-HIDROXIBUTIRATO
dc.subjectRESÍDUOS DE MACROALGAS
dc.subjectENGENHARIA BIOTECNOLÓGICA
dc.subjectALGAS
dc.subjectBIOTECHNOLOGY
dc.subjectGELIDIUM SESQUIPEDALE
dc.subjectHALOMONAS BOLIVIENSIS
dc.subjectSEAWEED RESIDUES
dc.subjectPOLY-3-HYDROXYBUTYRATE
dc.subjectMACROALGAE RESIDUES
dc.subjectBIOTECHNOLOGY ENGINEERING
dc.subjectALGAE
dc.titleUpgrading end-of-line residues of the red seaweed Gelidium sesquipedale to polyhydroxyalkanoates using Halomonas boliviensisen
dc.typearticle

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