Production, characterization, and immobilization of protease from the yeast Rhodotorula oryzicola

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dc.contributor.authorde Oliveira, Juliana Mota
dc.contributor.authorFernandes, Pedro
dc.contributor.authorBenevides, Raquel Guimarães
dc.contributor.authorde Assis, Sandra Aparecida
dc.contributor.institutionFE - Faculty of Engineering
dc.date.issued2021-10
dc.descriptionPublisher Copyright: © 2020 International Union of Biochemistry and Molecular Biology, Inc.
dc.description.abstractThe protease was produced extracellularly in submerged fermentation by the yeast Rhodotorula oryzicola using different sources of nitrogen and maximum activity (6.54 × 10−3 U/mg) was obtained in medium containing 2% casein (w/v). Purification of the protease by gel filtration chromatography resulted in a 3.07-fold increase of specific protease activity. The optimal pH and temperature for enzyme activity were 6.51 and 63.04 °C, respectively. Incubation in the presence of some salts enhanced enzyme activity, which peaked under 0.01 M BaCl2. The enzyme retained about 90% of enzymatic activity at temperatures 50–60 °C. The commercially available enzyme carriers evaluated, silica gel, Celite 545, and chitosan effectively immobilized the protease. The enzyme immobilized in Celite 545 retained 73.53% of the initial activity after 15 reuse cycles. These results are quite promising for large-scale production and immobilization of protease from R. oryzicola, as the high operational stability of the immobilized enzyme lowers production costs in biotechnological applications that require high enzymatic activity and stability under high temperatures.en
dc.identifier.citationde Oliveira, J M, Fernandes, P, Benevides, R G & de Assis, S A 2021, 'Production, characterization, and immobilization of protease from the yeast Rhodotorula oryzicola', Biotechnology and Applied Biochemistry, vol. 68, no. 5, pp. 1033-1043. https://doi.org/10.1002/bab.2023
dc.identifier.doihttps://doi.org/10.1002/bab.2023
dc.identifier.issn0885-4513
dc.identifier.urlhttps://www.scopus.com/pages/publications/85091315317
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley-Blackwell
dc.relation.ispartofBiotechnology and Applied Biochemistry
dc.rightsclosedAccess
dc.subjectBIOTECNOLOGIA
dc.subjectBIOQUÍMICA
dc.subjectENZIMOLOGIA
dc.subjectBIOTECHNOLOGY
dc.subjectBIOCHEMISTRY
dc.subjectENZYMOLOGY
dc.titleProduction, characterization, and immobilization of protease from the yeast Rhodotorula oryzicolaen
dc.typearticle

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