Inulinase from Rhodotorula mucilaginosa : immobilization and application in the production of fructooligosaccharides

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dc.contributor.authorde Araujo Ribeiro, Geise Camila
dc.contributor.authorFernandes, Pedro
dc.contributor.authorSilva, Dayse Alessandra Almeida
dc.contributor.authorBrandão, Hugo Neves
dc.contributor.authorde Assis, Sandra Aparecida
dc.contributor.institutionFaculdade de Engenharia
dc.date.issued2021-07
dc.descriptionPublisher Copyright: © 2021, The Korean Society of Food Science and Technology.
dc.description.abstractThe crude extract containing inulinase from Rhodotorula mucilaginosa was obtained by submerged fermentation. Inulinase was immobilized on chicken eggshell by physical adsorption and covalent crosslinking, using glutaraldehyde as a crosslinking reagent, and Celite by adsorption. Fructooligosaccharides production was performed using immobilized inulinase (5%, w/v) and inulin substrate solution under experimental conditions evaluated through Doehlert experimental design. The production of inulinase was optimized for concentrations of D-glucose and yeast extract at 12.5 and 0.5 g/L, respectively, resulting in an optimal activity of 0.62 U. The optimal pH and temperature for enzyme activity were 8.0 and 75 °C, respectively, leading to an optimal activity of 3.54 U. The highest immobilization efficiency (46.27%) was obtained upon immobilization on Celite. Immobilization by adsorption to eggshell allowed for specific activity of 4.15 U/g, and adsorption to Celite resulted in specific activity of 3.70 U/g. The highest titer in fructooligosaccharides was obtained with an initial inulin concentration of 250 g/L (25%, w/v), and a reaction time of 16 h. Hence, immobilized inulinase proved to be a promising catalyst for fructooligosaccharides production since the formulation is performed through a simple, low-cost, and large-scale applicable methodology.en
dc.description.sponsorshipThis work was supported by FINEP (Financier of Studies and Projects), CAPES (Coordination of Improvement of Higher Level Personnel), CNPq (National Research Council), and FAPESB (Foundation for Research Support of the State of Bahia). We also thank the Biotechnology Graduate Program of the State University of Feira de Santana (UEFS/FIOCRUZ).
dc.identifier.citationde Araujo Ribeiro, G C, Fernandes, P, Silva, D A A, Brandão, H N & de Assis, S A 2021, 'Inulinase from Rhodotorula mucilaginosa : immobilization and application in the production of fructooligosaccharides', Food Science and Biotechnology, vol. 30, no. 7, pp. 959-969. https://doi.org/10.1007/s10068-021-00931-x
dc.identifier.doihttps://doi.org/10.1007/s10068-021-00931-x
dc.identifier.issn1226-7708
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85107928677&partnerID=8YFLogxK
dc.language.isoeng
dc.peerreviewedyes
dc.publisherThe Korean Society of Food Science and Technology
dc.relation.ispartofFood Science and Biotechnology
dc.rightsclosedAccess
dc.subjectBIOTECNOLOGIA
dc.subjectBIOQUÍMICA
dc.subjectBIOQUÍMICA ALIMENTAR
dc.subjectBIOTECHNOLOGY
dc.subjectBIOCHEMISTRY
dc.subjectFOOD BIOCHEMISTRY
dc.titleInulinase from Rhodotorula mucilaginosa : immobilization and application in the production of fructooligosaccharidesen
dc.typearticle

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