Inulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharides

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dc.contributor.authorRibeiro, Geise Camila de Araújo
dc.contributor.authorFernandes, Pedro Carlos de Barros
dc.contributor.authorSilva, Dayse Alessandra Almeida
dc.contributor.authorBrandão, Hugo Neves
dc.contributor.authorAssis, Sandra Aparecida de
dc.date.accessioned2021-09-06T11:37:17Z
dc.date.available2021-09-06T11:37:17Z
dc.date.issued2021
dc.descriptionFood Science and Biotechnologypt
dc.description.abstractThe crude extract containing inulinase from Rhodotorula mucilaginosa was obtained by submerged fermentation. Inulinase was immobilized on chicken eggshell by physical adsorption and covalent crosslinking, using glutaraldehyde as a crosslinking reagent, and Celite by adsorption. Fructooligosaccharides production was performed using immobilized inulinase (5%, w/v) and inulin substrate solution under experimental conditions evaluated through Doehlert experimental design. The production of inulinase was optimized for concentrations of D-glucose and yeast extract at 12.5 and 0.5 g/L, respectively, resulting in an optimal activity of 0.62 U. The optimal pH and temperature for enzyme activity were 8.0 and 75 °C, respectively, leading to an optimal activity of 3.54 U. The highest immobilization efficiency (46.27%) was obtained upon immobilization on Celite. Immobilization by adsorption to eggshell allowed for specific activity of 4.15 U/g, and adsorption to Celite resulted in specific activity of 3.70 U/g. The highest titer in fructooligosaccharides was obtained with an initial inulin concentration of 250 g/L (25%, w/v), and a reaction time of 16 h. Hence, immobilized inulinase proved to be a promising catalyst for fructooligosaccharides production since the formulation is performed through a simple, low-cost, and large-scale applicable methodology.en
dc.description.sponsorshipFINEP (Financier of Studies and Projects), CAPES (Coordination of Improvement of Higher Level Personnel), CNPq (National Research Council), and FAPESB (Foundation for Research Support of the State of Bahia). Biotechnology Graduate Program of the State University of Feira de Santana (UEFS/FIOCRUZ).pt
dc.formatapplication/pdf
dc.identifier.other10.1007/s10068-021-00931-x
dc.identifier.urihttp://hdl.handle.net/10437/12146
dc.language.isoengpt
dc.publisherSpringerpt
dc.rightsopenAccess
dc.subjectBIOTECNOLOGIApt
dc.subjectBIOQUÍMICApt
dc.subjectBIOQUÍMICA ALIMENTARpt
dc.subjectBIOTECHNOLOGYen
dc.subjectBIOCHEMISTRYen
dc.subjectFOOD BIOCHEMISTRYen
dc.titleInulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharidespt
dc.typearticlept

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