Identificação por HPLC-MS dos polifenóis presentes num extrato de Myrtus communis L. obtido por extração supercrítica
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Data
2017
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Edições Universitárias Lusófonas
Resumo
Neste trabalho, estudámos um extrato obtido por extração de fluido supercrítica (ESC) usando um método mais
simples de adição de co-solvente (etanol). Em vez de usar uma bomba de líquidos, que é o processo mais comum,
o etanol foi introduzido diretamente na célula de extração, imediatamente após o carregamento da célula com a
amostra de planta. Pretendeu-se investigar se esta alteração teria algum efeito na composição do extrato obtido.
As condições experimentais utilizadas foram: temperatura 48 °C, pressão 10 MPa, caudal de fluido supercrítico
(FSC) 130,71 dm3h-1 (0,238 kgh-1) e um volume de etanol de 104 cm3. Os compostos identificados por HPLCMS neste extrato foram o ácido quínico, o ácido quínico 3,5-di-O-galato, o ácido 3,4,5-galoilquinico, a miricetinagalactósido-galato, a quercetina-galactósido-galato, a miricetina-galactósido e a quercetina-ramnósido).
In this work, we studied an extract obtained by supercritical fluid extraction (SFE) using a simpler method of cosolvent (ethanol) addition. Instead of using a liquid pump, which is the most common process, the ethanol was directly introduced in the extraction cell, immediately after loading the cell with the plant sample. it was our intent to investigate if this change would have any effect in the composition of the extract obtained. The experimental conditions used were: temperature 48° C, pressure 10 MPa, supercritical fluid (SCF) flow rate 130.71dm3h-1 (0.238 kgh-1) and an ethanol volume of 104 cm3. The composition of the extract obtained was different from previous tests, and the compounds identified by HPLC-MS were quinic acid, quinic acid 3,5-di-O-gallate, quinic acid 3,4,5-galloyl, myricetin-galactoside gallate, quercetin-galactoside gallate, quercetin, and myricetin-galactosiderhamnoside.
In this work, we studied an extract obtained by supercritical fluid extraction (SFE) using a simpler method of cosolvent (ethanol) addition. Instead of using a liquid pump, which is the most common process, the ethanol was directly introduced in the extraction cell, immediately after loading the cell with the plant sample. it was our intent to investigate if this change would have any effect in the composition of the extract obtained. The experimental conditions used were: temperature 48° C, pressure 10 MPa, supercritical fluid (SCF) flow rate 130.71dm3h-1 (0.238 kgh-1) and an ethanol volume of 104 cm3. The composition of the extract obtained was different from previous tests, and the compounds identified by HPLC-MS were quinic acid, quinic acid 3,5-di-O-gallate, quinic acid 3,4,5-galloyl, myricetin-galactoside gallate, quercetin-galactoside gallate, quercetin, and myricetin-galactosiderhamnoside.
Descrição
Biomedical and biopharmaceutical research : jornal de investigação biomédica e biofarmacêutica
Palavras-chave
MEDICINA, CIÊNCIAS FARMACÊUTICAS, PLANTAS MEDICINAIS, POLIFENÓIS, HPLC/MS, ETANOL, MEDICINE, PHARMACEUTICAL SCIENCES, MEDICINAL PLANTS, PHARMACEUTICAL SCIENCES, MEDICINAL PLANTS, POLYPHENOLS, HPLC/MS, ETHANOL