Acerca da medição da hidratação 'profunda' da pele
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Data
2009
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Edições Universitárias Lusófonas
Resumo
A água, na epiderme, deve distribuir-se de acordo com a estrutura histológica que se conhece para este tecido, admitindo-se que as células em diferenciação, desde a camada basal (100% de água) ao estrato córneo (cerca de 10% de água) possuam distintos conteúdos hídricos. O objectivo do presente estudo foi esclarecer, se métodos especialmente concebidos, como é o caso do sistema MoistureMeter- D®, conseguem detectar a água profunda da epiderme. Foram seleccionados 13 voluntários, nos quais se marcaram aleatoriamente 3 sítios de aplicação na face ventral do antebraço para aplicação de (a) um penso com a solução de Lauril Sulfato de Sódio (LSS) a 3,5%, (b) um penso sem qualquer solução (controlo positivo) e (c) uma área de controlo sem penso (controlo negativo). Após 24h, os pensos foram removidos, procedendo-se à medição da hidratação através de metodologias não invasivas, com o auxílio dos sistemas MoistureMeter-D® e MoistureMeter-SC®, sistemas baseados na medição da variação da “capacitância” epidérmica, utilizando diferentes frequências de medição, respectivamente, 300 MHz e 1,25MHz.A perda trans-epidérmica de água (PTEA) e o eritema, foram também medidos nos sítios estudados (Tewameter TM300 e Chromameter CR3000, respectivamente). Os dados foram estatisticamente avaliados (descritiva e não paramétrica) adoptando-seumnível de confiança de 95%. Os resultados obtidos revelam a alteração da função de barreira da pele e presença de edema no sítio tratado com LSS, conforme se encontra descrito na literatura. Em relação à medição da hidratação da epiderme, os resultados não evidenciaram diferenças significativas entre os sítios estudados, quando medidos pelo MoistureMeter-D®. Contudo, verificou-se uma maior hidratação superficial no local da aplicação do provocador, conforme se encontra descrito na literatura. Os presentes resultados sugerem que, apesar das especificações do fabricante, o sistema em causa não detecta as variações mais profundas de conteúdo hídrico descritas.
Epidermal water is distributed in accordance with the histological structure that is known for this tissue, admitting that the cells, from the basal layer (100% water) to the stratum corneum (approximately10%water) have distinct water contents. The objective of this study is to evaluate if methods specially conceived to measure deep skin water contents, such as the MoistureMeter-D®, can really do it. Thirteen volunteers participated in the study. Three sites on the ventral forearm were chosen and application, previously randomized, took place as follows: (a) test chamber with an aqueous solution of Sodium Lauryl Sulphate (SLS) (3.5%), (b) test chamber with no solution (positive control) and (c) one area with no test chamber (negative control). After 24 hours, the chambers were removed and several variables were measured by biometric techniques. Epidermal hydration was measured by “capacitance” based devices (MoistureMeter-D®and MoistureMeter-SC®), using different measure frequencies, 300MHzand 1.25 MHz, respectively, and Transepidermal water loss (TEWL) and erythema (Tewameter TM300 and Chromameter CR3000, respectively) were also measured. Results were statistically treated (descriptive and nonparametric), and a confidence level of 95%was adopted. Changes on skin barrier integrity and the presence of oedema in the SLS treated site were apparent, which is in agreement with what is described in the literature. Concerning the measurement of epidermal hydration, the results don't show any significant differences between the three sites, when measured by the MoistureMeter-D® system. However, higher levels of superficial hydration were detected on the site treated with SLS, as is also described in the literature. The present results suggest that, in spite of the manufacturer specifications, the system under scope, does not detect deeper changes on epidermis water content.
Epidermal water is distributed in accordance with the histological structure that is known for this tissue, admitting that the cells, from the basal layer (100% water) to the stratum corneum (approximately10%water) have distinct water contents. The objective of this study is to evaluate if methods specially conceived to measure deep skin water contents, such as the MoistureMeter-D®, can really do it. Thirteen volunteers participated in the study. Three sites on the ventral forearm were chosen and application, previously randomized, took place as follows: (a) test chamber with an aqueous solution of Sodium Lauryl Sulphate (SLS) (3.5%), (b) test chamber with no solution (positive control) and (c) one area with no test chamber (negative control). After 24 hours, the chambers were removed and several variables were measured by biometric techniques. Epidermal hydration was measured by “capacitance” based devices (MoistureMeter-D®and MoistureMeter-SC®), using different measure frequencies, 300MHzand 1.25 MHz, respectively, and Transepidermal water loss (TEWL) and erythema (Tewameter TM300 and Chromameter CR3000, respectively) were also measured. Results were statistically treated (descriptive and nonparametric), and a confidence level of 95%was adopted. Changes on skin barrier integrity and the presence of oedema in the SLS treated site were apparent, which is in agreement with what is described in the literature. Concerning the measurement of epidermal hydration, the results don't show any significant differences between the three sites, when measured by the MoistureMeter-D® system. However, higher levels of superficial hydration were detected on the site treated with SLS, as is also described in the literature. The present results suggest that, in spite of the manufacturer specifications, the system under scope, does not detect deeper changes on epidermis water content.
Descrição
Revista Lusófona de Ciências e Tecnologias da Saúde
Palavras-chave
MEDICINA, DERMATOLOGIA, HIDRATAÇÃO, PELE, BIOMETRIA, EDEMA, ESTUDOS DE CASO, DISPOSITIVOS MÉDICOS, LAURIL SULFATO DE SÓDIO, MEDICINE, DERMATOLOGY, HYDRATION, SKIN, BIOMETRICS, CASE STUDIES, MEDICAL DEVICES, SODIUM LAURYL SULPHATE, EDEMA
Citação
Rosado , C , Raimundo , H & Rodrigues , L M 2009 , ' Acerca da medição da hidratação 'profunda' da pele ' , Revista Lusófona de Ciências e Tecnologias da Saúde .