Avaliação da citotoxicidade in vitro dos ácidos ferulico, cafeico e p-cumárico em células renais tumorais humanas
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2020
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Edições Universitárias Lusófonas
Resumo
Os compostos bioativos provenientes de produtos naturais têm sido amplamente estudados como potenciais agentes
terapêuticos, devido às suas propriedades químicas, estruturais, e biológicas. De entre estes, os ácidos hidroxicinâmicos
emergem com um potencial farmacológico muito interessante para prevenir e tratar várias doenças, incluindo o cancro. No
entanto, enquanto alguns estudos demonstram a sua atividade antitumoral, outros estudos indicam o oposto. Uma vez que o
Impacto desses compostos no cancro renal não está bem estabelecido, são necessários mais estudos para compreender melhor
os seus efeitos biológicos e os seus potenciais usos terapêuticos neste cancro.
Neste trabalho, a citotoxicidade de três ácidos hidroxicinâmicos: ácidos ferúlico, cafeico e p-cumárico foi avaliada em células
tumorais renais humanas 786-0, através dos ensaios do MTT e do CV. Nenhum dos compostos naturais estudados, até 250 uM,
mostrou efeitos citotóxicos na linha celular 786-0. A concentrações acima de 250 uM, enquanto que o ácido ferúlico não
influenciou significativamente a viabilidade celular das células 786-0, os ácidos p-cumárico e cafeico diminuiram a viabilidade
das células renais tumorais. No entanto, novos estudos sobre a atividade biológica destes compostos, utilizando outras
metodologias, bem como outros modelos celulares tumorais, devem ser realizados.
Bioactive compounds from natural sources have been extensively studied as potential therapeutic agents, due to their chemical, structural, and biological properties. Among them, hydroxycinnamic acids emerge with a very interesting pharmacological potential to prevent and treat several diseases, including cancer. However, while some studies support their anticancer activity, other reports indicate otherwise. Since the impact of these compounds on renal cancer 1s not well established, further studies are needed to better understand their biological effects and their potential therapeutic uses in this cancer. In this work, the cytotoxicity of three hydroxycinnamic acids: ferulic, caffeic and p-coumaric acids, was evaluated on 786-O0 human renal cancer cells by MTT and CV assays. At concentrations up to 250 uM, none of the studied natural compounds showed cytotoxic effects to 786-0 cell line. At higher concentrations (500 and 1000 uM), while ferulic acid also did not significantly influence the cell viability of 786-0 cells, p-coumaric and caffeic acids decreased the renal cancer cells viability. Nevertheless, further studies on the biological activity of these compounds, using other methodologies, as well as using other cancer cell models, should be performed.
Bioactive compounds from natural sources have been extensively studied as potential therapeutic agents, due to their chemical, structural, and biological properties. Among them, hydroxycinnamic acids emerge with a very interesting pharmacological potential to prevent and treat several diseases, including cancer. However, while some studies support their anticancer activity, other reports indicate otherwise. Since the impact of these compounds on renal cancer 1s not well established, further studies are needed to better understand their biological effects and their potential therapeutic uses in this cancer. In this work, the cytotoxicity of three hydroxycinnamic acids: ferulic, caffeic and p-coumaric acids, was evaluated on 786-O0 human renal cancer cells by MTT and CV assays. At concentrations up to 250 uM, none of the studied natural compounds showed cytotoxic effects to 786-0 cell line. At higher concentrations (500 and 1000 uM), while ferulic acid also did not significantly influence the cell viability of 786-0 cells, p-coumaric and caffeic acids decreased the renal cancer cells viability. Nevertheless, further studies on the biological activity of these compounds, using other methodologies, as well as using other cancer cell models, should be performed.
Descrição
Biomedical and biopharmaceutical research : jornal de investigação biomédica e biofarmacêutica
Palavras-chave
MEDICINA, TOXICIDADE, MEDICINE, TOXICITY, ÁCIDO FERÚLICO, ÁCIDOS CAFEÍCOS, ÁCIDO P-CUMÁRICO, FERULIC ACID, CAFFEIC ACIDS, P-COUMARIC ACID, ÁCIDOS HIDROXICINÂMICOS, HYDROXYCINNAMIC ACIDS