Microfluidic bioreactors for enzymatic synthesis in packed-bed reactors—Multi-step reactions and upscaling
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Data
2020-11-10
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Elsevier B.V.
Resumo
Enzymatic synthesis of biochemical commodities is of upmost importance as it represents a greener alternative to traditional chemical synthesis and provides easier downstream processing strategies compared to fermentation-based processes. A microfluidic system used to optimize the enzymatic production of both levodopa (L-DOPA) and dopamine in both single-step and multistep-reaction sequences with yield of approximately 30 % for L-DOPA production and 70 % for dopamine production is presented. The system for L-DOPA production was then up-scaled (780-fold increase) to a milliliter scale system by maintaining similar mass transport properties resulting in the same yield, space-time yield and biocatalyst yield as its microscale counterpart. The results obtained for yield and biocatalyst yield (351.7 mgL-DOPA mg−1Tyr h−1) were similar to what is reported in the literature for similar systems, however the space-time yield (0.806 mgL-DOPA L−1 h−1) was smaller. This work demonstrates a microfluidic bioreactor that can be used for complex optimizations that can be performed rapidly while reducing the consumption of reagents by immobilizing the catalyst on a carrier which can then be used in a packed-bed reactor, thus extending the enzyme life span.
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Publisher Copyright: © 2020 Elsevier B.V.
Palavras-chave
Biocatalysis, Cascade reactions, Dopamine, L-DOPA, Microfluidics, Packed-bed reactor
Citação
Brás, E J S, Domingues, C, Chu, V, Fernandes, P & Conde, J P 2020, 'Microfluidic bioreactors for enzymatic synthesis in packed-bed reactors—Multi-step reactions and upscaling', Journal of Biotechnology, vol. 323, pp. 24-32. https://doi.org/10.1016/j.jbiotec.2020.07.016