Microfluidic bioreactors for enzymatic synthesis in packed-bed reactors—Multi-step reactions and upscaling

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dc.contributor.authorBrás, Eduardo J.S.
dc.contributor.authorDomingues, Cristiana
dc.contributor.authorChu, Virginia
dc.contributor.authorFernandes, Pedro
dc.contributor.authorConde, João Pedro
dc.contributor.institutionHEI-LAB - Human Environment Interaction Lab
dc.date.issued2020-11-10
dc.descriptionPublisher Copyright: © 2020 Elsevier B.V.
dc.description.abstractEnzymatic synthesis of biochemical commodities is of upmost importance as it represents a greener alternative to traditional chemical synthesis and provides easier downstream processing strategies compared to fermentation-based processes. A microfluidic system used to optimize the enzymatic production of both levodopa (L-DOPA) and dopamine in both single-step and multistep-reaction sequences with yield of approximately 30 % for L-DOPA production and 70 % for dopamine production is presented. The system for L-DOPA production was then up-scaled (780-fold increase) to a milliliter scale system by maintaining similar mass transport properties resulting in the same yield, space-time yield and biocatalyst yield as its microscale counterpart. The results obtained for yield and biocatalyst yield (351.7 mgL-DOPA mg−1Tyr h−1) were similar to what is reported in the literature for similar systems, however the space-time yield (0.806 mgL-DOPA L−1 h−1) was smaller. This work demonstrates a microfluidic bioreactor that can be used for complex optimizations that can be performed rapidly while reducing the consumption of reagents by immobilizing the catalyst on a carrier which can then be used in a packed-bed reactor, thus extending the enzyme life span.en
dc.identifier.citationBrás, E J S, Domingues, C, Chu, V, Fernandes, P & Conde, J P 2020, 'Microfluidic bioreactors for enzymatic synthesis in packed-bed reactors—Multi-step reactions and upscaling', Journal of Biotechnology, vol. 323, pp. 24-32. https://doi.org/10.1016/j.jbiotec.2020.07.016
dc.identifier.doihttps://doi.org/10.1016/j.jbiotec.2020.07.016
dc.identifier.issn0168-1656
dc.identifier.urlhttps://www.scopus.com/pages/publications/85088659109
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Biotechnology
dc.rightsopenAccess
dc.subjectBiocatalysis
dc.subjectCascade reactions
dc.subjectDopamine
dc.subjectL-DOPA
dc.subjectMicrofluidics
dc.subjectPacked-bed reactor
dc.titleMicrofluidic bioreactors for enzymatic synthesis in packed-bed reactors—Multi-step reactions and upscalingen
dc.typearticle

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