Microfluidic bioreactors for enzymatic synthesis in packed-bed reactors—Multi-step reactions and upscaling

Ver registo simples
dc.contributor.authorBrás, Eduardo João Silva
dc.contributor.authorDomingues, Cristiana
dc.contributor.authorChu, Virgínia
dc.contributor.authorFernandes, Pedro Carlos de Barros
dc.contributor.authorConde, João Pedro
dc.date.accessioned2021-09-06T11:21:07Z
dc.date.available2021-09-06T11:21:07Z
dc.date.issued2020
dc.descriptionJournal of Biotechnologypt
dc.description.abstractEnzymatic synthesis of biochemical commodities is of upmost importance as it represents a greener alternative to traditional chemical synthesis and provides easier downstream processing strategies compared to fermentation-based processes. A microfluidic system used to optimize the enzymatic production of both levodopa (L-DOPA) and dopamine in both single-step and multistep-reaction sequences with yield of approximately 30 % for L-DOPA production and 70 % for dopamine production is presented. The system for L-DOPA production was then up-scaled (780-fold increase) to a milliliter scale system by maintaining similar mass transport properties resulting in the same yield, space-time yield and biocatalyst yield as its microscale counterpart. The results obtained for yield and biocatalyst yield (351.7 mgL-DOPA mg−1Tyr h−1) were similar to what is reported in the literature for similar systems, however the space-time yield (0.806 mgL-DOPA L−1 h−1) was smaller. This work demonstrates a microfluidic bioreactor that can be used for complex optimizations that can be performed rapidly while reducing the consumption of reagents by immobilizing the catalyst on a carrier which can then be used in a packed-bed reactor, thus extending the enzyme life spanen
dc.description.sponsorshipFundação para a Ciência e a Técnologia (FCT) for funding through a PhD grant for E.J.S. Bras within the AIM Doctoral Program (PD/BD/ 128167/2016), the research projects OptLoC (PTDC/BBB-NAN/5927/ 2014) and the Research Units (UID/NAN/50024 and UID/Multi/ 04046/2013). Funding for the project POINT4PAC (SAICTPAC/0019/ 2015 and LISBOA-01-0145-FEDER-016405) was through European Structural & Investment Funds through the COMPETE Programme and from National Funds through FCT – Fundação para a Ciência e a Tecnologiapt
dc.formatapplication/pdf
dc.identifier.other10.1016/j.jbiotec.2020.07.016
dc.identifier.urihttp://hdl.handle.net/10437/12145
dc.language.isoengpt
dc.publisherElsevierpt
dc.rightsopenAccess
dc.subjectBIOTECNOLOGIApt
dc.subjectBIOQUÍMICApt
dc.subjectENZIMASpt
dc.subjectDOPAMINApt
dc.subjectLEVODOPApt
dc.subjectBIOTECHNOLOGYen
dc.subjectBIOCHEMISTRYen
dc.subjectENZYMESen
dc.subjectDOPAMINEen
dc.subjectLEVODOPAen
dc.titleMicrofluidic bioreactors for enzymatic synthesis in packed-bed reactors—Multi-step reactions and upscalingpt
dc.typearticlept

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
Microfluidic bioreactors for enzymatic synthesis in packed-bed reactors—Multi-step reactions and upscaling.pdf
Tamanho:
3.31 MB
Formato:
Adobe Portable Document Format
Descrição:
paper published
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

FE - Artigos de Revistas Internacionais com Arbitragem Científica