Microfluidic bioreactors for enzymatic synthesis in packed-bed reactors—Multi-step reactions and upscaling

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dc.contributor.authorBrás, Eduardo João Silva
dc.contributor.authorDomingues, Cristiana
dc.contributor.authorChu, Virgínia
dc.contributor.authorFernandes, Pedro Carlos de Barros
dc.contributor.authorConde, João Pedro
dc.contributor.institutionFaculdade de Engenharia
dc.date.issued2020
dc.descriptionJournal of Biotechnology
dc.description.abstractEnzymatic synthesis of biochemical commodities is of upmost importance as it represents a greener alternative to traditional chemical synthesis and provides easier downstream processing strategies compared to fermentation-based processes. A microfluidic system used to optimize the enzymatic production of both levodopa (L-DOPA) and dopamine in both single-step and multistep-reaction sequences with yield of approximately 30 % for L-DOPA production and 70 % for dopamine production is presented. The system for L-DOPA production was then up-scaled (780-fold increase) to a milliliter scale system by maintaining similar mass transport properties resulting in the same yield, space-time yield and biocatalyst yield as its microscale counterpart. The results obtained for yield and biocatalyst yield (351.7 mgL-DOPA mg−1Tyr h−1) were similar to what is reported in the literature for similar systems, however the space-time yield (0.806 mgL-DOPA L−1 h−1) was smaller. This work demonstrates a microfluidic bioreactor that can be used for complex optimizations that can be performed rapidly while reducing the consumption of reagents by immobilizing the catalyst on a carrier which can then be used in a packed-bed reactor, thus extending the enzyme life spanen
dc.description.statusNon peer reviewed
dc.formatapplication/pdf
dc.identifier.citationBrás , E J S , Domingues , C , Chu , V , Fernandes , P C D B & Conde , J P 2020 , ' Microfluidic bioreactors for enzymatic synthesis in packed-bed reactors—Multi-step reactions and upscaling ' , Default journal .
dc.identifier.issn1646-3730
dc.language.isoeng
dc.publisherEdições Universitárias Lusófonas
dc.relation.ispartofDefault journal
dc.rightsopenAccess
dc.subjectBIOTECNOLOGIA
dc.subjectBIOQUÍMICA
dc.subjectENZIMAS
dc.subjectDOPAMINA
dc.subjectLEVODOPA
dc.subjectBIOTECHNOLOGY
dc.subjectBIOCHEMISTRY
dc.subjectENZYMES
dc.subjectDOPAMINE
dc.subjectLEVODOPA
dc.titleMicrofluidic bioreactors for enzymatic synthesis in packed-bed reactors—Multi-step reactions and upscalingen

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