CBIOS - Posters

URI permanente para esta coleção:https://hdl.handle.net/10437/11231

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Quality-by-design optimization of CBD nanoemulsions : incorporating choline-based ionic liquids as sustainable surfactants
(Reunião Anual do Colégio de Indústria da Ordem dos Farmacêuticos, 2025-10-03) Lopez, Yolanda; Gomes, Ana P.; Rocha, Miguel; Rosatella, Andreia; Rijo, Patricia; Pereira-Leite, Catarina; Costa, Maria do Céu; Oliveira, Manuel J.; CBIOS - Research Center for Biosciences & Health Technologies
A CBD-loaded NE was prepared using a choline-based IL as a stabilizing agent, with the same composition as the optimized formulation but replacing conventional surfactants by the IL: 1.5% MCT, 1.75% CBD and 1% IL. The formulation displayed a droplet size of approximately 125 nm and a low PDI of 0.156. After 24 hours, the NE retained its integrity, showing only slight variations in particle size and PDI, while maintaining a stable zeta potential of -38 mV and pH of 10.1. These findings indicate that choline oleate IL can serve as a promising alternative to conventional surfactants, providing comparable emulsifying performance. Despite IL-based systems typically yielding droplet sizes, promoting enhanced bioavailability and improved resistance to oxidation and hydrolysis due to the protective nature of the IL matrix in contrast to Span 80/Tween 80 nanoemulsions that tend to form larger droplets and show reduced stability under oxidative stress, the innovation and sustainability potential of NE-ILs still requires toxicological validation and regulatory clearance before large-scale implementation. Further studies are needed to evaluate its long-term stability and biopharmaceutical potential.
Engineering TransfersomIL Nanocarriers to improve topical delivery of caffeic acid
(Training School: Nanoparticle Development for Skin Drug Delivery, 2026) Martins, Marta Filipa de Seixas Barata; Júlio, Ana; Saraiva, Nuno; Rosado, Catarina; Pereira-Leite, Catarina; CBIOS - Research Center for Biosciences & Health Technologies
Evaluation of the functional and rheological properties of grape pomace flour with different particle sizes
(The 6th International Electronic Conference on Foods, 2025) Gonçalves, Elsa M.; Gonçalves, Diogo; Fradinho, Patrícia; Gomes, Sandra; Alvarenga, Nuno; Nicolai, Marisa; Palma, Maria Lídia; Pereira, Paula; EPCV - School of Psychology and Life Sciences; CBIOS - Research Center for Biosciences & Health Technologies
Grape pomace, a by-product of the winemaking industry, contains nutritionally relevant compounds, including proteins, lipids, carbohydrates, phenolic compounds, vitamins, and dietary fibers. Given its functional and technological potential, this by-product has been valorized as a flour for use in food formulations. The particle size of the flour directly influences its physicochemical and rheological properties, affecting its performance in food matrices. This study evaluated the functionality of grape pomace flour with different particle sizes. Samples were subjected to drying, milling, and sieving, followed by the analysis of water absorption capacity (WAC; g water/g flour), oil absorption capacity (OAC; g oil/g flour), milk absorption capacity (MAC; g milk/g flour), water solubility (%), moisture retention capacity (g/g), and rheological properties (M.A.R.S iQ Air Rheometer, Thermo Scientific), including viscosity (Pa·s), elasticity, and flow behavior (Pa). The results showed that the flour exhibited a high absorption capacity for all the tested solvents (water, oil, and milk), with values exceeding 1.5 g solvent/g flour, indicating strong potential for retention and interaction with both polar and non-polar components. These properties are particularly relevant for applications in food products requiring emulsifying stability. The gelling capacity was more pronounced at ratios of 1:1.5 and 1:2 (flour-to-water), with a significant increase in viscosity, suggesting the formation of a cohesive three-dimensional structure. Rheological analyses revealed a viscoelastic behavior suitable for structured food systems such as gels, emulsions, and moldable doughs. Moreover, samples with finer particle sizes showed improved functional performance, indicating greater technological potential for incorporation into value-added food formulations with specific texture and stability requirements.
Food applications of grape pomace : nutritional, antioxidant, and functional insights
(The 6th International Electronic Conference on Foods, 2025) Mendes, Gonçalo; Lageiro, Manuela; Palma, Maria Lídia; Pereira, Paula; Nicolai, Marisa; CBIOS - Research Center for Biosciences & Health Technologies
The wine industry, a major player in the global agri-food sector, generates millions of tonnes of grape pomace annually as a by-product. Although rich in polyphenols, fiber, and antioxidants with proven health benefits, most pomace remains underutilized, often being discarded or repurposed as low-value compost or animal feed. This study highlights the untapped potential of grape pomace flour by analyzing its bioactive profile, emphasizing its viability for high-value applications in nutraceuticals, functional foods, and pharmaceuticals. Six samples from two Portuguese grape varieties (Arinto and Touriga Nacional) were sourced from three wineries (Carmim, Cerrado da Porta, Mingorra) and analyzed. Total phenolic content (114.94–128.31 mg GAE/100 g) and flavonoids (452.74–2,214.27 mg quercetin equivalents/100 g) were quantified spectrophotometrically, demonstrating potent antioxidant activity (FRAP: 110.56– 543.00 µmol FeSO₄/100 g; DPPH: 586.93–698.71 µmol Trolox/100 g). Dietary fiber stood out (45.29–64.97%), surpassing many conventional sources, while HPLC analysis confirmed Bvitamins and calculated macronutrient profiles. The findings reveal grape pomace as a sustainable, nutrient-dense ingredient. In food production, it could enrich baked goods, snacks, or supplements, leveraging its fiber for gut health and its polyphenols for oxidative stress reduction. Cosmetic applications include anti-aging serums, harnessing their free radical-scavenging properties. Pharmaceutical uses extend to antiinflammatory formulations or controlled-release delivery systems, with emerging research supporting its role in mitigating metabolic syndrome and cognitive decline. By transforming waste into value-added products, this approach aligns with circular economy principles, reducing environmental impact while creating new revenue streams for wineries. Further research should optimize extraction methods and evaluate bioavailability to maximize commercial adoption.
Evaluation of the impact of IL on SLNs with Gelucire® or Precirol ATO®
(1st International Electronic Conference on Medicinal Chemistry and Pharmaceutics, 2025) Júlio, Ana; ALMEIDA, CINTIA JESUS; Vieira, João Manuel Barreto; Martins, Marta Filipa de Seixas Barata; ROQUE, ROSSANA MIRIAM GOULÃO; Rosado, Catarina; Pereira-Leite, Catarina; CBIOS - Research Center for Biosciences & Health Technologies
Nanosystems are gradually being more commonly used in a variety of applications, providing a new approach to therapeutics by improving the pharmacokinetic profile of several compounds. This has led to an increase in the search for bioinspired and sustainable materials for novel nanotechnology-based formulations with pharmaceutical and cosmetic purposes. For the delivery of bioactives to the skin, lipid nanoparticles, such as solid lipid nanoparticles (SLNs), have been thoroughly investigated. Their use is still being hampered, nevertheless, by issues with stability and drug loading during storage. Several methodologies, including the applicability of ionic liquids (ILs), may be used to improve this. These have proven to be a potential strategy due to the beneficial qualities that our group identified in earlier work, namely their capacity to enhance the colloidal stability of formulations. Using two different commercial lipids (GelucireR 43/01 vs. Precirol ATOR 5) and an amino acid-based IL (2-hydroxyethyl)-trimethylammonium-L-phenylalaninate [Cho][Phe], this study aimed to assess the impact of the incorporation of the IL into the SLN, comparing both commercial lipids. After conducting stability tests and characterising these nanosystems in terms of size, zeta potential, and polydispersity index, SLNs with favourable properties were produced. The findings demonstrated that ILs helped to stabilise the nanoparticles and enhance their physicochemical characteristics for topical use. All things considered, choline-based ILs in combination with the creation of novel lipid nanocarriers from sustainable and bioinspired materials appear to create a new paradigm for skin delivery. These findings demonstrate that ILs can alter SLN size, which might enhance their physicochemical characteristics for topical use.
Efeito entourage em canábis medicinal : potencial terapêutico e limitações atuais
(Reunião Anual do Colégio de Indústria da Ordem dos Farmacêuticos, 2025-10-05) Gomes, Ana Patrícia; André, Rebeca; Pereira-Leite, Catarina; Costa, António Marques da; Rodrigues, Luís Monteiro; Rijo, Patricia; Costa, Maria do Céu; Sassano, Michael; CBIOS (FCT) - Centro de Investigação em Biociências e Tecnologias da Saúde
Biotech Agrifood Innovation : a pre-acceleration program and contest to promote knowledge and technology transfer in agri-food and agri-environmental sectors
(3º Bio Iberoamérica – Congresso Ibero-Americano de Biotecnologia, 2022-04-07) OLIVEIRA, LEANDRO JORGE GUIMARÃES DE; Santos, Telmo; Cardoso, Eduardo Luís; CBIOS - Research Center for Biosciences & Health Technologies
The European agri-food sector needs to overcome several related to sustainability and food security [1]. For this, it is necessary for professionals working in this area to be able toinnovate, and to propose solutions that must be based on scientific knowledge, and on models of lifelong and postgraduate education, which allow the efficient transfer of knowledge between industry and higher education institutions [2]. This study aims to develop and implement a contest and pre-acceleration program for business projects in the agri-food and agrienvironment areas through academic entrepreneurship.
Self-assembly nanoparticles of 7α-acetoxy-6β-hydroxyroyleanone isolated from Plectranthus hadiensis
(6th International Electronic Conference on Medicinal Chemistry, 2020-11-05) MARTIN, EVA MARIA DOMINGUEZ; Ntungwe, Epole; Isca, Vera Mónica Sousa; Colombo, Eleonora; Pinto, Tatiana; Pacheco, Catarina Alves; Coelho, Jaime A. S.; Bangay, Gabrielle; Díaz-Lanza, Ana María; Passarella, Daniele; Rijo, Patricia; CBIOS - Research Center for Biosciences & Health Technologies
Plectranthus genus belongs to the Lamiaceae family and consists of around 300 species distributed from Africa to Asia and Australia. Plectranthus spp. has been reported to be rich in diterpenoids, such as different types of royleanones. One of Plectranthus medicinal plants species is P. hadiensis (Forssk.) Schweinf. ex Sprenger, which contains diverse metabolites such as phenolic and diterpenoid compounds. Furthermore, this plant is widely used in an array of traditional and Ayurvedic recipes for the treatment of diverse range of ailments like cancer.
Nanoparticle systems for improved delivery of plectranthus-derived abietane diterpenoids in breast cancer therapy
(1st International Electronic Conference on Medicinal Chemistry and Pharmaceutics, 2025-11-01) Isca, Vera M. S.; Bangay, Gabrielle; Lopes, Elizabeth A.; André, Rebeca; Alarcón, Jennifer Fernández; Ntungwe, Epole; Reis, Catarina; Díaz-Lanza, Ana María; Puigvert, Cristina; Rijo, Patrícia; CBIOS - Research Center for Biosciences & Health Technologies
Nanoparticles (NPs) have become key tools in modern drug delivery, especially when used alongside natural compounds. This combination can improve how drugs are absorbed and directed to specific targets in the body. Benefits include higher drug loading, controlled and sustained release, longer presence in the bloodstream, and reduced side effects. These advantages contribute to safer and more efficient therapies. Among the various nanoparticle types, gold nanoparticles (AuNPs) stand out due to their easy production, strong stability, and unique physical and chemical traits. These properties make them excellent carriers for both drugs and genetic material, especially in cancer-related applications. One group of natural compounds with promising anticancer activity is the abietane diterpenoids, such as 7α-acetoxy-6β-hydroxyroyleanone (Roy), found in Plectranthus species. Despite their potential, their clinical use is limited by poor water solubility and low bioavailability. To overcome these issues, Roy and one Roy-esterified derivative were incorporated into gold nanoparticle-based systems. These nanoformulations were evaluated for characteristics like particle size (29.37 nm), polydispersity index (PDI, 0.399), surface charge (zeta potential, −6.84), and drug encapsulation efficiency (79.4%). In vitro experiments using breast cancer cell lines (MDA-MB-231, 4T1, and MCF7) showed that Roy-loaded AuNPs had significantly stronger anticancer effects than Roy alone or unmodified AuNPs. Notably, these systems also demonstrated selectivity, affecting cancer cells while sparing healthy human dermal fibroblasts (HDFs), suggesting strong potential for targeted cancer therapy. These results represent meaningful progress in the development of innovative drug delivery systems for cancer therapy.

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