Estudo do potencial efeito antitumoral do ácido 4-hidroxi-4-O-sulfato benzóico no carcinoma renal humano
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2023
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O cancro, incluindo o cancro renal, é uma doença complexa multietapas e multifatorial. O subtipo mais frequente é o carcinoma renal celular, que corresponde a cerca de 90% dos casos de cancro renal. Para além das opções terapêuticas utilizadas, tem-se procurado tratamentos e combinações mais eficazes e seguros. Nesse sentido, surgiu a aposta emergente no estudo de compostos bioativos presentes na dieta, e nos seus metabolitos. Estes compostos têm se revelado promissores pelas suas diversas propriedades, entre as quais antitumorais. Uma classe de compostos bioativos com particular interesse é a classe dos polifenóis. Assim, nesta dissertação, pretendeu-se realizar uma revisão da literatura relativamente ao cancro e ao cancro renal, com especial foco na etiologia, fatores de risco e principais opções terapêuticas disponíveis. Foram igualmente descritos os compostos bioativos, bem como as suas principais classes e os seus potenciais efeitos terapêuticos. Este trabalho incluiu uma parte experimental, onde se avaliou potencial efeito antitumoral do ácido 4-hidroxi-4-O-sulfato-benzóico (BAOS) utilizando um modelo celular in vitro de adenocarcinoma renal humano (células 786-O), quer isoladamente, quer em associação com a cisplatina, através de ensaios de viabilidade celular – cristal violeta – e de ensaios de migração celular, através do ensaio de Wound Healing. Os resultados mostraram que o BAOS, nas condições experimentais utilizadas, não teve um impacto significativo na viabilidade e na migração celular coletiva das células 786-O, nem de forma isolada, nem em combinação com a cisplatina. No entanto, é importante continuar a estudar este composto e outros semelhantes, através de condições experimentais diferentes e realizando outros ensaios complementares. Palavras-chave: cancro renal, 786-O, ácido 4-hidroxi-4-O-sulfato-benzóico, viabilidade celular, cristal violeta, migração celular coletiva
Cancer, including kidney cancer, is a complex disease with multiple stages and multiple factors. The most frequent subtype is the renal cell carcinoma, with 90% of the total kidney cancer cases. Beyond the therapeutic options that have been used, the search of treatments and combinations with better efficacy and security has been made. With this thought in mind, the study of bioactive compounds from diet, and its metabolites, has been rising. These compounds have been shown promising results because of its various proprieties, such as the antitumoral capacity. An example of a class with particular interest is the polyphenols class. In this dissertation, a literature review was made about cancer and kidney cancer, focusing in the etiology, risk factors and main therapeutic options that are available. The bioactive compounds were described, as well as the main classes and potential therapeutic effects. This work included an experimental part, where the antitumoral effect potential of the 4-(sulfooxy)benzoic acid (BAOS) was evaluated, by using an in vitro cell model of human renal adenocarcinoma (786-O cells), either alone, either in a combination with cisplatin, by the viability assays – crystal violet – and cell migration assays, by the wound healing assay. The results showed that BAOS, in the used conditions, didn’t have a strong impact either in the viability, either in the collective cell migration of 786-O cells, either alone, either in a combination with cisplatin. Nevertheless, it is important to keep studying this compound and others similar, using different experimental conditions and using other complement assays. Keywords: kidney cancer, 786-O, 4-(sulfooxy)benzoic acid, cell viability, crystal-violet, collective cell migration
Cancer, including kidney cancer, is a complex disease with multiple stages and multiple factors. The most frequent subtype is the renal cell carcinoma, with 90% of the total kidney cancer cases. Beyond the therapeutic options that have been used, the search of treatments and combinations with better efficacy and security has been made. With this thought in mind, the study of bioactive compounds from diet, and its metabolites, has been rising. These compounds have been shown promising results because of its various proprieties, such as the antitumoral capacity. An example of a class with particular interest is the polyphenols class. In this dissertation, a literature review was made about cancer and kidney cancer, focusing in the etiology, risk factors and main therapeutic options that are available. The bioactive compounds were described, as well as the main classes and potential therapeutic effects. This work included an experimental part, where the antitumoral effect potential of the 4-(sulfooxy)benzoic acid (BAOS) was evaluated, by using an in vitro cell model of human renal adenocarcinoma (786-O cells), either alone, either in a combination with cisplatin, by the viability assays – crystal violet – and cell migration assays, by the wound healing assay. The results showed that BAOS, in the used conditions, didn’t have a strong impact either in the viability, either in the collective cell migration of 786-O cells, either alone, either in a combination with cisplatin. Nevertheless, it is important to keep studying this compound and others similar, using different experimental conditions and using other complement assays. Keywords: kidney cancer, 786-O, 4-(sulfooxy)benzoic acid, cell viability, crystal-violet, collective cell migration
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Orientação: João Guilherme Costa
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MESTRADO INTEGRADO EM CIÊNCIAS FARMACÊUTICAS, CIÊNCIAS FARMACÊUTICAS, PHARMACEUTICAL SCIENCES, CANCRO RENAL, KIDNEY CANCER, COMPOSTOS FITOQUÍMICOS, PHYTOCHEMICALS