Impacto de compostos polifenólicos na progressão do cancro renal
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2023
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O carcinoma renal das células claras e o tipo mais comum de cancro renal em adultos compreendendo até 80 % dos tumores renais. As abordagens terapêuticas variam de acordo com o estádio do cancro. As principais opções para o tratamento são a cirurgia e o tratamento farmacológico, nomeadamente imunoterapia ou terapêuticas dirigidas. De forma a complementar ou otimizar as terapêuticas existentes, têm sido realizados vários estudos com compostos de origem natural, presentes em alimentos, com o objetivo de explorar o seu potencial. Nos últimos anos, vários estudos têm vindo a demonstrar as vantagens dos polifenóis em diversas doenças pelas suas diversas atividades, tais como anticarcinogénica e antioxidante. No entanto, o impacto dos polifenóis no cancro renal não está bem estabelecido, sendo necessários mais estudos para compreender melhor o seu potencial. Tendo em consideração a farmacocinética desfavorável e a extensa metabolização dos compostos polifenolicos, e útil verificar a Acão dos seus metabolitos a nível renal para avaliar uma potencial acão anticancerígena. Deste modo, o presente estudo teve como objetivo estudar o impacto de diferentes metabolitos de compostos polifenólicos no cancro renal. Para tal, realizou-se uma revisão bibliográfica sobre as propriedades farmacocinéticas dos compostos polifenolicos, a sua presença na dieta, os seus metabolitos e precursores e utilizaram-se bases de dados como Phenol-Hub e Phenol Explorer. Adicionalmente, estudou-se "in vitro" o impacto de vários metabolitos de compostos polifenolicos na imigração coletiva das células 786-O de cancro renal humano, através do ensaio de cicatrização da ferida (Wound-Healing). Os resultados do estudo experimental demonstraram que os compostos mais promissores foram os ácidos homovanílico, vanílico e protocatecuico, uma vez que foram aqueles que mais reduziram a migração celular. Com este trabalho foi possível verificar a relação entre diversos metabolitos de compostos polifenolicos e o desenvolvimento do cancro renal. Contudo, são necessários mais estudos, sobre os efeitos anticancerígenos desta classe de compostos, incluindo os seus metabolitos. Palavras-Chave: Cancro renal, células 786-O, polifenóis, metabolitos, migração celular, ensaio de Wound-Healing
Renal clear cell carcinoma is the most common type of renal cancer in adults comprising up to 80% of renal tumors. Therapeutic approaches vary according to the stage of the cancer. The main options for treatment are surgery and pharmacological treatment, namely immunotherapy or targeted therapies. In order to complement or improve existing therapies, several studies were carried out with compounds of natural origin, present in foods, with the aim of exploring their potential. In recent years, several studies have demonstrated the advantages of polyphenols in various diseases due to their various activities, such as anticarcinogenic and antioxidant. However, the impact of polyphenols on kidney cancer is not well established, and more studies are needed to better understand their potential. Considering the unfavorable pharmacokinetics and extensive metabolization of polyphenolic compounds, it is useful to verify the action of their metabolites at the renal level to assess a potential anticancer action. Thus, the present study aimed to identify the impact of different metabolites of polyphenolic compounds on kidney cancer. To achieve this, a literature review was carried out on the pharmacokinetic properties of phenolic compounds, their presence in the diet, their metabolites, and precursors, and databases such as Phenol-Hub and Phenol Explorer were used. In addition, the impact of various metabolites of polyphenolic compounds on the collective migration of human kidney cancer 786-O cells was studied in vitro using the Wound-Healing Assay. The results of the experimental study showed that the most promising compounds were homovanillic, vanillic and protocatechuic acids since they were the ones that most reduced cell migration. With this work it was possible to verify a relationship between several metabolites of polyphenolic compounds and the development of kidney cancer. Nevertheless, it is necessary to carry out further studies on the anticancer effects of this class of compounds, including their metabolites. Keywords: Renal cancer, 786-O cells, polyphenols, metabolites, cell migration, Wound- Healing Assay
Renal clear cell carcinoma is the most common type of renal cancer in adults comprising up to 80% of renal tumors. Therapeutic approaches vary according to the stage of the cancer. The main options for treatment are surgery and pharmacological treatment, namely immunotherapy or targeted therapies. In order to complement or improve existing therapies, several studies were carried out with compounds of natural origin, present in foods, with the aim of exploring their potential. In recent years, several studies have demonstrated the advantages of polyphenols in various diseases due to their various activities, such as anticarcinogenic and antioxidant. However, the impact of polyphenols on kidney cancer is not well established, and more studies are needed to better understand their potential. Considering the unfavorable pharmacokinetics and extensive metabolization of polyphenolic compounds, it is useful to verify the action of their metabolites at the renal level to assess a potential anticancer action. Thus, the present study aimed to identify the impact of different metabolites of polyphenolic compounds on kidney cancer. To achieve this, a literature review was carried out on the pharmacokinetic properties of phenolic compounds, their presence in the diet, their metabolites, and precursors, and databases such as Phenol-Hub and Phenol Explorer were used. In addition, the impact of various metabolites of polyphenolic compounds on the collective migration of human kidney cancer 786-O cells was studied in vitro using the Wound-Healing Assay. The results of the experimental study showed that the most promising compounds were homovanillic, vanillic and protocatechuic acids since they were the ones that most reduced cell migration. With this work it was possible to verify a relationship between several metabolites of polyphenolic compounds and the development of kidney cancer. Nevertheless, it is necessary to carry out further studies on the anticancer effects of this class of compounds, including their metabolites. Keywords: Renal cancer, 786-O cells, polyphenols, metabolites, cell migration, Wound- Healing Assay
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Orientação: João Guilherme Costa
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MESTRADO INTEGRADO EM CIÊNCIAS FARMACÊUTICAS, CIÊNCIAS FARMACÊUTICAS, PHARMACEUTICAL SCIENCES, CANCRO RENAL, KIDNEY CANCER, POLIFENÓIS, POLYPHENOLS