Quercetina, um flavonóide com potencial impacto no cancro renal?
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Data
2022
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Resumo
O cancro é uma doença que tem vindo a afetar cada vez mais pessoas em todo o
mundo. Caracterizado pelo crescimento anormal das células, desrespeitando o seu ciclo e
crescimento natural. É sabido através de agências de estatística que a incidência do cancro
renal, em particular, tem vindo a aumentar, sendo um dos tipos de cancro mais frequentes
em Portugal. No que respeita ao seu tratamento, têm vindo a ser realizadas investigações na
tentativa de descobrir novos tratamentos farmacológicos, de forma a aumentar a sobrevida
dos doentes. Neste sentido, têm sido exploradas novas classes de compostos, como alguns
compostos bioativos presentes na dieta, os quais têm revelado propriedades interessantes,
como a ação antitumoral. A quercetina, pertencente à classe dos flavonóides é um desses
compostos bioativos com diversas propriedades, incluindo essa atividade antitumoral.
Neste sentido, no âmbito desta dissertação estudou-se experimentalmente o impacto
da quercetina no cancro renal. Para isso, foi utilizado como modelo «in vitro», as células
786-O, células tumorais renais humanas e o ensaio do cristal violeta para avaliar o impacto
deste composto na viabilidade celular (entre 24 e 48 h). A quercetina foi testada em
diferentes concentrações (1,5; 2,5; 5; 7,5 e 10 µM) isoladamente e posteriormente em
combinação com a cisplatina (25 µM), fármaco antitumoral amplamente utilizado.
A quercetina não demonstrou efeito antitumoral, nas condições experimentais e no
modelo celular utilizados. No entanto, deverão ser realizados mais estudos e continuar a
avaliar-se o seu potencial contra o cancro renal
Cancer is a disease that has been affecting more and more people all over the world. Characterized by the abnormal growth of cells, disrespecting their cycle and natural growth. It is known through statistical agencies that the incidence of renal cancer, in particular, has been increasing, being one of the most frequent types of cancer in Portugal. As regards its treatment, research has been carried out in an attempt to discover new pharmacological treatments to increase patient survival. In this sense, new classes of compounds have been explored, such as some bioactive compounds present in the diet, which have revealed interesting properties, such as antitumour action. Quercetin, belonging to the flavonoid class, is one of these bioactive compounds with several properties, including antitumour activity. In the scope of this dissertation the impact of quercetin on kidney cancer was experimentally studied. To this end, 786-O cells, human renal tumour cells, were used as an "in vitro" model and the crystal violet assay was used to evaluate the impact of the compounds on cell viability (between 24 and 48 h). Quercetin was tested at different concentrations (1.5; 2.5; 5; 7.5 and 10 µM) alone and subsequently in combination with cisplatin (25 µM), a widely used antitumour drug. Quercetin showed no antitumour effect under the experimental conditions and in the cell model used. However, further studies should be carried out and its potential against renal cancer should continue to be evaluated.
Cancer is a disease that has been affecting more and more people all over the world. Characterized by the abnormal growth of cells, disrespecting their cycle and natural growth. It is known through statistical agencies that the incidence of renal cancer, in particular, has been increasing, being one of the most frequent types of cancer in Portugal. As regards its treatment, research has been carried out in an attempt to discover new pharmacological treatments to increase patient survival. In this sense, new classes of compounds have been explored, such as some bioactive compounds present in the diet, which have revealed interesting properties, such as antitumour action. Quercetin, belonging to the flavonoid class, is one of these bioactive compounds with several properties, including antitumour activity. In the scope of this dissertation the impact of quercetin on kidney cancer was experimentally studied. To this end, 786-O cells, human renal tumour cells, were used as an "in vitro" model and the crystal violet assay was used to evaluate the impact of the compounds on cell viability (between 24 and 48 h). Quercetin was tested at different concentrations (1.5; 2.5; 5; 7.5 and 10 µM) alone and subsequently in combination with cisplatin (25 µM), a widely used antitumour drug. Quercetin showed no antitumour effect under the experimental conditions and in the cell model used. However, further studies should be carried out and its potential against renal cancer should continue to be evaluated.
Descrição
Orientação: João Guilherme Costa ; Coorientação: Ana Sofia Fernandes
Palavras-chave
MESTRADO INTEGRADO EM CIÊNCIAS FARMACÊUTICAS, CIÊNCIAS FARMACÊUTICAS, CANCRO RENAL, CISPLATINA, CÉLULAS, CITOTOXICIDADE, PHARMACEUTICAL SCIENCES, KIDNEY CANCER, CISPLATIN, CELLS, CYTOTOXICITY