Actividades inibitória de colinesterases e antioxidante de extractos de plantas utilizadas como chás
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2012
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Nos últimos séculos perdeu-se o interesse por uso de plantas medicinais nos países desenvolvidos, devido à evolução da síntese química de fármacos. Ressurgiu recentemente este uso para desenvolvimento de novos fármacos de patologias com múltiplos mecanismos fisiopatológicos associados devido às actividades simultâneas dos extractos.
Dos potenciais usos medicinais explorou-se a inibição da acetilcolinesterase (AChE) para problemas gastrointestinais e para terapêutica da doença de Alzheimer com menos efeitos secundários, e menor toxicidade associada.
Realizou-se um estudo de actividades antioxidante e inibitória de AChE onde as duas espécies estudadas de plantas tiveram actividade. A decocção de A. cherimola apresentou capacidade de actividade mais elevada de inibir Ache com um IC50 de 0,627±0,028 mg/mL e antioxidante com um EC50 de 15,435±0,184 mg/mL. Nenhum dos extractos perdeu actividade com o metabolismo in vitro gástrico e pancreático.
Na biodisponibilidade verificou-se que a maior parte dos compostos permeia até determinada extensão a monocamada de células Caco-2 com excepção do glucósido da quercetina. A via de transporte predominante dos compostos estudados parece ser difusão paracelular passiva.
In the last centuries the use of medicinal plants in the developed countries was lost due to the progress of drug chemical synthesis. It surfaced recently because of research and development of new drugs for diseases with multiple causes, since plant extracts seem to have simultaneous actions. From the potential medicinal uses, the inhibition of AChE was explored to treat gastrointestinal problems and for Alzheimer’s disease with less side effects and toxicity. A study of the inhibition of AChE and antioxidant activity was made, where the two species of plants showed activity. The highest activity in AChE inhibition was from A. cherimola decoction with an IC50 of 0,627±0,028 mg/mL and antioxidant activity with an EC50 of 15,435±0,184 mg/mL. None of the extracts lost activity after gastric and pancreatic in vitro metabolism. In the bioavailability, most of the compounds pass through to a certain extent the Caco-2 cell monolayer with the exception of the quercetin glucoside. The dominant transport pathway for the studied compounds appears to be paracellular passive diffusion.
In the last centuries the use of medicinal plants in the developed countries was lost due to the progress of drug chemical synthesis. It surfaced recently because of research and development of new drugs for diseases with multiple causes, since plant extracts seem to have simultaneous actions. From the potential medicinal uses, the inhibition of AChE was explored to treat gastrointestinal problems and for Alzheimer’s disease with less side effects and toxicity. A study of the inhibition of AChE and antioxidant activity was made, where the two species of plants showed activity. The highest activity in AChE inhibition was from A. cherimola decoction with an IC50 of 0,627±0,028 mg/mL and antioxidant activity with an EC50 of 15,435±0,184 mg/mL. None of the extracts lost activity after gastric and pancreatic in vitro metabolism. In the bioavailability, most of the compounds pass through to a certain extent the Caco-2 cell monolayer with the exception of the quercetin glucoside. The dominant transport pathway for the studied compounds appears to be paracellular passive diffusion.
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Orientação : Maria de Fátima Frazão, Maria Luísa Serralheiro
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DOENÇA DE ALZHEIMER, ALZHEIMER'S DISEASE, MESTRADO INTEGRADO EM CIÊNCIAS FARMACÊUTICAS, MEDICAMENTOS, DRUGS, PLANTAS MEDICINAIS, MEDICINAL PLANTS, CIÊNCIAS FARMACÊUTICAS, PHARMACEUTICAL SCIENCES