Quantifying myocardial fibrosis on murine model using ImageJ

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dc.contributor.authorCatarino, José Carlos Mota
dc.contributor.authorViegas, Inês
dc.contributor.authorRodrigues, Joana
dc.contributor.authorPinto, Marta
dc.contributor.authorBota, Gonçalo
dc.contributor.authorCravo, Francisco
dc.contributor.authorLúcio, Filipa
dc.contributor.authorFaísca, Pedro
dc.contributor.institutionFaculdade de Medicina Veterinária
dc.date.issued2019
dc.descriptionRevista Lusófona de Ciência e Medicina Veterinária
dc.description.abstractQuantification of histological images is a task often required in biomedical research. Many of the methods used are time-consuming, difficult to replicate and subjective. This work aimed to describe and validate a more objective, reproducible and easy to perform methodology to measure murine heart fibrosis, using ImageJ software. For this matter, a macro was created and validated for its intra and inter-observational reproducibility. No significant differences were found between the results obtained manually and with the macro (p> 0.05). Regarding the interobserver variability, the macro proved to be a less variable method by presenting higher correlation coefficients between observers when compared with the manual measurement. With these results, we were able to define and describe a methodology that outputs objective and replicable results in a less laborious way.en
dc.description.statusNon peer reviewed
dc.formatapplication/pdf
dc.identifier.citationCatarino , J C M , Viegas , I , Rodrigues , J , Pinto , M , Bota , G , Cravo , F , Lúcio , F & Faísca , P 2019 , ' Quantifying myocardial fibrosis on murine model using ImageJ ' , Revista Lusófona de Ciência e Medicina Veterinária . https://doi.org/10.60543/rlcmv.v9i0.6639
dc.identifier.doihttps://doi.org/10.60543/rlcmv.v9i0.6639
dc.identifier.issn1646-8805
dc.language.isoeng
dc.publisherEdições Universitárias Lusófonas
dc.relation.ispartofRevista Lusófona de Ciência e Medicina Veterinária
dc.rightsopenAccess
dc.subjectIMAGEJ
dc.subjectVETERINÁRIA
dc.subjectMEDICINA VETERINÁRIA
dc.subjectFIBROSE
dc.subjectCORAÇÃO
dc.subjectMORFOMETRIA
dc.subjectPATOLOGIA EXPERIMENTAL
dc.subjectFIBROSIS
dc.subjectHEART
dc.subjectVETERINARY MEDICINE
dc.subjectMORPHOMETRY
dc.subjectEXPERIMENTAL PATHOLOGY
dc.titleQuantifying myocardial fibrosis on murine model using ImageJen
dc.typearticle

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Revista Lusófona de Ciência e Medicina Veterinária Vol. 09 (2018)