Revista Lusófona de Ciência e Medicina Veterinária Vol. 09 (2018)
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Item Infecção por micoplasmas hemotrópicos felinos numa colónia de gatos errantes da Ilha de Faro(Edições Universitárias Lusófonas, 2019) Alves, Marta dos Santos Ferreira; Alves, Maria Margarida Ferreira; Faculdade de Medicina VeterináriaHemoplasmosis is a disease caused by ubiquitous bacterial agents, called hemotropic mycoplasmas, that infect erythrocytes of several mammals and are cause of hemolytic anemia. There are three species that may infect cats – Mycoplasma haemofelis, “Candidatus Mycoplasma haemominutum” and “Candidatus Mycoplasma turicensis” –presenting different degrees of pathogenicity. The present study aimed to determine, by molecular techniques, the presence of infection caused by these agents in a colony of 157 stray cats from Faro Island. DNA samples, isolated from peripheral blood of the tested animals, were submitted to PCR to amplify a 16S rDNA gene fragment of Mycoplasma spp.. PCR products were, then, submitted to restriction enzyme hydrolysis or DNA sequencing to identify the infecting species. In parallel, the studied population was categorized by age, sex and presence of ectoparasites; potential relationships between Mycoplasma spp. infection and epidemiological variables were also investigated. A percentage of 20.4% positive PCR results was obtained. The percentage for the different species was, in order of decreasing percentage: 7% for “Candidatus Mycoplasma haemominutum” single infection, followed by Mycoplasma haemofelis with 4.46% and “Candidatus Mycoplasma turicensis” in 1.72% of samples. Mixed infection of Mycoplasma haemofelis and “Candidatus Mycoplasma haemominutum” was found in 7.64% of the animals. Statistical analysis showed a significant association between Mycoplasma spp. infection and being male or adult. The present work contributed to increase the knowledge about hemotropic mycoplasmas infection in stray cats from Faro Island, not only in relation to their distribution and different infecting species, but also to its associated risk factors.Item Caracterização da ocorrência de parasitas gastrointestinais de gatos na zona de pesca da Ilha de Faro(Edições Universitárias Lusófonas, 2019) Duque de Araújo, Ana Munoz; Caldas, Mariana; Faculdade de Medicina VeterináriaThe recent strategic interests and expectations created in the sector of exploration of marine resources, among them are the traditional activities, especially artisanal fishing, makes certain areas of the country that have this tradition contribute the economical and socio-cultural valorisation of the marine resources and the interests of these extremely important sector for the Algarve. The Association of Street Animals (AAR) developed the Faro Beach Project that aimed to control the populations of stray dogs and cats. A total of 33 fecal samples were collected, representing 27% of the animals submitted to veterinary treatments. The coprological techniques used were qualitative, Willis Flotation and Natural Sedimentation techniques. The parasitism was observed in 60.6% of the animals (20/33), and mixed infections were detected in 45% (9/20). Parasitism by helminths of the genus Toxocara cati was observed in 70% of the samples, (14/20), followed by Ancylostoma spp. 35% (7/20), Dipylidium caninum 5% (1/20) and protozoan oocysts of Cystoisospora felis were observed in 35% (7/20) of the animals. The results showed the high degree of parasitism in the cats of this colony, in particular the occurrence of zoonotic agents such as Toxocara cati. The cohabitation of these animals with the human population and the proximity to fish collection areas may favour the transmission of parasites with zoonotic potential.Item Quantifying hepatic fibrosis on murine models: how to obtain representative results in a less laborious way(Edições Universitárias Lusófonas, 2019) Catarino, José Carlos Mota; Viegas, Inês; Rodrigues, Joana; Pinto, Marta; Faísca, Pedro; Faculdade de Medicina VeterináriaIn biomedical research, quantification of histological images is often required. Many of the methods used are time-consuming, laborious, originate variable results and are difficult to replicate. This paper is aimed towards finding a more objective, reproducible and easy to perform method to obtain representative results in murine models of hepatic fibrosis using ImageJ software. To do so on a liver fibrosis model, the percentages of fibrotic lesion obtained in an entire section and in several different magnifications were compared. No statistically significant differences were found (p> 0.05), but the correlation was stronger between the results obtained in the entire section photograph and the two photographs at 40x (r = 0.963). Using ImageJ facilitated the definition of a methodology that originated representative results on a liver section and at the same time allowed objective measurement in a reproducible and less laborious way.Item Quantifying myocardial fibrosis on murine model using ImageJ(Edições Universitárias Lusófonas, 2019) Catarino, José Carlos Mota; Viegas, Inês; Rodrigues, Joana; Pinto, Marta; Bota, Gonçalo; Cravo, Francisco; Lúcio, Filipa; Faísca, Pedro; Faculdade de Medicina VeterináriaQuantification of histological images is a task often required in biomedical research. Many of the methods used are time-consuming, difficult to replicate and subjective. This work aimed to describe and validate a more objective, reproducible and easy to perform methodology to measure murine heart fibrosis, using ImageJ software. For this matter, a macro was created and validated for its intra and inter-observational reproducibility. No significant differences were found between the results obtained manually and with the macro (p> 0.05). Regarding the interobserver variability, the macro proved to be a less variable method by presenting higher correlation coefficients between observers when compared with the manual measurement. With these results, we were able to define and describe a methodology that outputs objective and replicable results in a less laborious way.