Criopreservação de espermatozóides de garanhões recuperados da cauda do epidídimo
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2018
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A criopreservação de espermatozóides através da cauda do epidídimo é uma
técnica que tem vindo a ser muito utilizada, uma vez que permite recolher material
genético. Deste modo é possível a preservação de espécies, animais ameaçados de
extinção, animais com lesões ou doenças graves ou morte inesperada. Apesar de ainda
não estar completamente estudada esta técnica tem sido uma mais valia na preservação
de sémen de garanhões com elevado valor genético.
Este estudo tem por objectivo a colheita de espermatozóides através da cauda
do epidídimo e acompanhar o seu processo de criopreservação, avaliando
posteriormente os parâmetros espermáticos pós-descongelamento e, demonstrar que
esses parâmetros são significativamente superiores aos obtidos através de sémen
colhido por Vagina Artificial.
Para esse efeito realizou-se a recolha de espermatozóides através da Cauda do
Epidídimo de três garanhões pós-orquiectomia, pela técnica de fluxo retrógrado com os
diluidores e crioprotectores apropriados e de seguida, foi realizado o processo de
criopreservação. Após o congelamento e posteriormente o descongelamento das
palhetas foram avaliados os parâmetros espermáticos de anteriores colheitas de sémen
por Vagina Artificial com o sistema CASA e compararam-se os valores com os obtidos
através da Cauda do Epidídimo.
Verificaram-se os parâmetros de motilidade total, motilidade progressiva,
concentração e número de espermatozóides para a Vagina Artificial e Cauda do
Epidídimo, tendo-se concluído que os parâmetros espermáticos para a Cauda do
Epidídimo são sempre superiores.
Conclui-se que a criopreservação de espermatozóides de garanhões
recuperados da cauda do epidídimo, é a técnica que melhores resultados tem na
preservação de material genético, sugerindo-se que a mesma continue a ser estudada e
promova avanços na reprodução equina.
Cryopreservation of sperm through the tail of the epididymis is a method that has been widely used, asit is the latest genetic material to ensure the preservation of species with difficulties in semen collection, of endangered animals, of animals with serious injury or diseases, or unexpected death. Although it has not yet been fully studied, this method has been an advantage in the preservation of semen of stallions with high genetic value. This study aims to collect sperm through the tail of the epididymis and its cryopreservation, to subsequently evaluating the post-thawing sperm parameters, and to demonstrate that these parameters are significantly higher than those obtained through semen collected by Artificial Vagina. For this purpose, spermatozoa were collected through the Tail of the Epididymis of three stallions after orchiectomy, using the retrograde flow technique with appropriate diluents and cryoprotectants. The cryopreservation process was also performed. After the thawing of the straws, the sperm parameters of previous s were collected using Artificial Vagina with the CASA system. The values were then compared with those obtained through the Epididymis Tail. The parameters of total motility, progressive motility, concentration and number of spermatozoa for the Artificial Vagina and Tail of the Epididym were verified, and it was concluded that the spermatic parameters for the Tail of the Epididymis are always higher. Summing up, cryopreservation of spermatozoa from stallions recovered from the tail of the epididymis, is the technique that best preserves genetic material. This conclusion suggests that this technique should continue to be studied and promote advances in equine reproduction.
Cryopreservation of sperm through the tail of the epididymis is a method that has been widely used, asit is the latest genetic material to ensure the preservation of species with difficulties in semen collection, of endangered animals, of animals with serious injury or diseases, or unexpected death. Although it has not yet been fully studied, this method has been an advantage in the preservation of semen of stallions with high genetic value. This study aims to collect sperm through the tail of the epididymis and its cryopreservation, to subsequently evaluating the post-thawing sperm parameters, and to demonstrate that these parameters are significantly higher than those obtained through semen collected by Artificial Vagina. For this purpose, spermatozoa were collected through the Tail of the Epididymis of three stallions after orchiectomy, using the retrograde flow technique with appropriate diluents and cryoprotectants. The cryopreservation process was also performed. After the thawing of the straws, the sperm parameters of previous s were collected using Artificial Vagina with the CASA system. The values were then compared with those obtained through the Epididymis Tail. The parameters of total motility, progressive motility, concentration and number of spermatozoa for the Artificial Vagina and Tail of the Epididym were verified, and it was concluded that the spermatic parameters for the Tail of the Epididymis are always higher. Summing up, cryopreservation of spermatozoa from stallions recovered from the tail of the epididymis, is the technique that best preserves genetic material. This conclusion suggests that this technique should continue to be studied and promote advances in equine reproduction.
Descrição
Orientação: Manuel Pequito ; co-orientação: Maria Gaivão Sepúlveda
Palavras-chave
MESTRADO INTEGRADO EM MEDICINA VETERINÁRIA, VETERINÁRIA, MEDICINA VETERINÁRIA, CRIOPRESERVAÇÃO DE SÉMEN, CAVALOS, ESTUDOS DE CASO, VETERINARY MEDICINE, SEMEN CRYOPRESERVATION, HORSES, CASE STUDIES