Avaliação da expressão de reguladores ósseos (RANKL) em neoplasias odontogénicas caninas: estudo piloto
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2019
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As neoplasias odontogénicas têm origem nos tecidos embrionários associados à formação do dente e do periodonto. O ameloblastoma acantomatoso canino (AAC) e o fibroma odontogénico periférico (FOP) são as duas neoplasias odontogénicas mais comuns da cavidade oral do cão. Ambos têm um carácter biológico benigno e não metastizam. Contudo, o AAC é localmente agressivo, podendo causar lise do osso adjacente.
O objetivo deste estudo foi caraterizar a expressão do RANKL através da técnica de imunohistoquímica (IHC), um regulador de reabsorção óssea, nestes dois tipos de tumores. As amostras examinadas incluíram 40 FOPs e sete AACs provenientes de diferentes cães. Verificou-se uma possível expressão RANKL positiva em todos os AACs estudados. Somente 27% dos FOP demonstraram marcação.
Estes resultados demonstram a importância da expressão do RANKL no AAC, dado que todas as amostras se terem revelado positivas como esperado. No FOP foram obtidos resultados positivos, contudo, estes não poderão ser considerados, uma vez que se detetaram problemas na avaliação destas lâminas e que inviabilizam a obtenção de possíveis conclusões.
Consideramos que os problemas detetados na realização do ensaio poderiam ter sido corrigidos através da alteração e otimização da técnica de IHC realizada, deixando-se, no entanto, identificados os pontos a alterar por forma a permitir que no futuro o conhecimento sobre a expressão de RANKL em tumores odontogénicos caninos possa ser ampliado.
Odontogenic tumors are neoplasms derived from ectodermal or mesenchymal components of the tooth forming apparatus. Canine acanthomatous ameloblastoma (CAA) and peripheral odontogenic fibroma (POF) are the two most representative odontogenic neoplasms that affect the oral cavity of dogs. Both have a benign biological character and don’t metastasize However, CAA is very aggressive locally and lysis of the adjacent bone might occur. The aim of this study was to characterize the expression of bone resorption regulators (RANKL) using the immunohistochemistry technique of both types of tumors. Samples of 40 POF and seven CAA tissues from canine patients were examined. RANKL expression was positively expressed in all CAA cases. Only 27% of POF cases demonstrated staining. These results show the importance of the RANKL expression in the CAA due to all the samples having positive results, as expected. Since POF had positive results, these will not be considered significant, because that problems during the observation of the slide were detected and it made it impossible to draw any conclusions. The problems detected during the experiment could be corrected by changing and optimizing the IHC protocol, however, all points that need adjustments are identified in order to allow the increased knowledge of RANKL in odontogenic tumors in dogs in the future
Odontogenic tumors are neoplasms derived from ectodermal or mesenchymal components of the tooth forming apparatus. Canine acanthomatous ameloblastoma (CAA) and peripheral odontogenic fibroma (POF) are the two most representative odontogenic neoplasms that affect the oral cavity of dogs. Both have a benign biological character and don’t metastasize However, CAA is very aggressive locally and lysis of the adjacent bone might occur. The aim of this study was to characterize the expression of bone resorption regulators (RANKL) using the immunohistochemistry technique of both types of tumors. Samples of 40 POF and seven CAA tissues from canine patients were examined. RANKL expression was positively expressed in all CAA cases. Only 27% of POF cases demonstrated staining. These results show the importance of the RANKL expression in the CAA due to all the samples having positive results, as expected. Since POF had positive results, these will not be considered significant, because that problems during the observation of the slide were detected and it made it impossible to draw any conclusions. The problems detected during the experiment could be corrected by changing and optimizing the IHC protocol, however, all points that need adjustments are identified in order to allow the increased knowledge of RANKL in odontogenic tumors in dogs in the future
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Orientação: Daniel
Murta, João Filipe Requicha, Inês Veiga
Palavras-chave
MESTRADO INTEGRADO EM MEDICINA VETERINÁRIA, VETERINÁRIA, MEDICINA VETERINÁRIA, NEOPLASIAS, CANÍDEOS, CÃES, CAVIDADE ORAL, VETERINARY MEDICINE, NEOPLASMS, CANIDS, DOGS, ORAL CAVITY, REABSORÇÃO ÓSSEA, BONE RESORPTION