Impacto de ácidos hidroxicinâmicos alimentares na progressão do cancro renal
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2021
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Os compostos fenólicos, compostos bioativos naturais, têm sido amplamente estudados como potenciais agentes anticancerígenos devido às suas capacidades para modular mecanismos redox e inflamatórios. No entanto é necessário realizar estudos de forma a corroborar a ação anticancerígena destes compostos e, ainda, determinar as concentrações a que estes exercem efeito.
O presente trabalho tem como objetivo a avaliação da citotoxicidade in vitro dos ácidos p-cumárico e ferúlico em células renais tumorais humanas (786-O).
Neste estudo avaliou-se a citotoxicidade de dois compostos fenólicos, os ácidos p-cumárico e ferúlico, a concentrações de 10 μM e 20 μM, isoladamente e em combinação com a cisplatina, nas células 786-O. Esta avaliação foi feita pelo método colorimétrico Cristal Violeta.
Os ácidos p-cumárico e ferúlico a concentrações de 10 μM não produziram efeito citotóxico na linha celular utilizada. No entanto, a 20 μM revelaram uma possível atividade antitumoral, ainda que pouco significativa, a qual foi corroborada na presença de cisplatina. Este estudo deverá continuar a ser aprofundado, de forma a considerar futuramente os benefícios da combinação de compostos fenólicos como agentes anti-cancerígenos.
Phenolic compounds, natural bioactive compounds, have been widely studied as potential anticancer agents due to their ability to modulate redox and inflammatory pathways. However, it is necessary to carry out studies in order to corroborate the anticancer action of these compounds and also to determine the concentrations at which they exert an effect. The present work aims to evaluate the in vitro cytotoxicity of p-coumaric and ferulic acids in human renal tumor cells (786-O). In this study, was evaluated the cytotoxicity of two phenolic compounds, p-coumaric and ferulic acids, at concentrations of 10 μM and 20 μM, both isolated and combined with cisplatin, in 786-O cells. This evaluation was carried out by using the Violet Crystal colorimetric method. The p-coumaric and ferulic acids at concentrations of 10 μM did not produce cytotoxic effect in the cell line used. However, at 20 μM they revealed a possible antitumoral activity, although not very significant, which was corroborated in the presence of cisplatin. This study should continue to be deepened, in order to consider the benefits of the combination of phenolic compounds as anti-cancer agents in the future.
Phenolic compounds, natural bioactive compounds, have been widely studied as potential anticancer agents due to their ability to modulate redox and inflammatory pathways. However, it is necessary to carry out studies in order to corroborate the anticancer action of these compounds and also to determine the concentrations at which they exert an effect. The present work aims to evaluate the in vitro cytotoxicity of p-coumaric and ferulic acids in human renal tumor cells (786-O). In this study, was evaluated the cytotoxicity of two phenolic compounds, p-coumaric and ferulic acids, at concentrations of 10 μM and 20 μM, both isolated and combined with cisplatin, in 786-O cells. This evaluation was carried out by using the Violet Crystal colorimetric method. The p-coumaric and ferulic acids at concentrations of 10 μM did not produce cytotoxic effect in the cell line used. However, at 20 μM they revealed a possible antitumoral activity, although not very significant, which was corroborated in the presence of cisplatin. This study should continue to be deepened, in order to consider the benefits of the combination of phenolic compounds as anti-cancer agents in the future.
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Orientação: Ana Sofia Fernandes ; coorientação: João Guilherme Costa
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MESTRADO INTEGRADO EM CIÊNCIAS FARMACÊUTICAS, CIÊNCIAS FARMACÊUTICAS, COMPOSTOS FENÓLICOS, CITOTOXICIDADE, CANCRO RENAL, PHARMACEUTICAL SCIENCES, PHENOLIC COMPOUNDS, CYTOTOXICITY, KIDNEY CANCER, ÁCIDOS HIDROXICINÂMICOS, HYDROXYCINNAMIC ACIDS