FE - Artigos de Revistas Internacionais com Arbitragem Científica

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Percorrer FE - Artigos de Revistas Internacionais com Arbitragem Científica por autor "Carvalho, Carla C. C. R. de"

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    Multi-enzyme systems in flow chemistry
    (Edições Universitárias Lusófonas, 2021) Fernandes, Pedro Carlos de Barros; Carvalho, Carla C. C. R. de; Faculdade de Engenharia
    Recent years have witnessed a growing interest in the use of biocatalysts in flow reactors. This merging combines the high selectivity and mild operation conditions typical of biocatalysis with enhanced mass transfer and resource efficiency associated to flow chemistry. Additionally, it provides a sound environment to emulate Nature by mimicking metabolic pathways in living cells and to produce goods through the systematic organization of enzymes towards efficient cascade reactions. Moreover, by enabling the combination of enzymes from different hosts, this approach paves the way for novel pathways. The present review aims to present recent developments within the scope of flow chemistry involving multi-enzymatic cascade reactions. The types of reactors used are briefly addressed. Immobilization methodologies and strategies for the application of the immobilized biocatalysts are presented and discussed. Key aspects related to the use of whole cells in flow chemistry are presented. The combination of chemocatalysis and biocatalysis is also addressed and relevant aspects are highlighted. Challenges faced in the transition from microscale to industrial scale are presented and discussed
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    Mycobacterium vaccae adaptation to disinfectants and hand sanitisers, and evaluation of cross-tolerance with antimicrobials
    (Edições Universitárias Lusófonas, 2020) Carvalho, Carla C. C. R. de; Teixeira, Raquel; Fernandes, Pedro Carlos de Barros; Faculdade de Engenharia
    Mycobacterium vaccae is being considered as an adjuvant to antituberculosis therapy, tested for the treatment of autoimmune diseases, and as an anti-depressive agent. This bacterium is ubiquitous in the environment and the widespread use of disinfectants and sanitisers may lead to its adaptation to these compounds. In the present study, M. vaccae cells adapted to these compounds mainly by making adjustments in their lipid composition and net surface charge. The modifications in the lipid composition led to changes in membrane permeability which resulted in increased tolerance towards levofloxacin, thioridazine, and omeprazole.