Quantifying hepatic fibrosis on murine models: how to obtain representative results in a less laborious way
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Data
2019
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Editora
Edições Universitárias Lusófonas
Resumo
In biomedical research, quantification of histological images is often required. Many of the methods used are time-consuming, laborious, originate variable results and are difficult to replicate. This paper is aimed towards finding a more objective, reproducible and easy to perform method to obtain representative results in murine models of hepatic fibrosis using ImageJ software. To do so on a liver fibrosis model, the percentages of fibrotic lesion obtained in an entire section and in several different magnifications were compared. No statistically significant differences were found (p> 0.05), but the correlation was stronger between the results obtained in the entire section photograph and the two photographs at 40x (r = 0.963).
Using ImageJ facilitated the definition of a methodology that originated representative results on a liver section and at the same time allowed objective measurement in a reproducible and less laborious way.
Descrição
Revista Lusófona de Ciência e Medicina Veterinária
Palavras-chave
IMAGEJ, VETERINÁRIA, MEDICINA VETERINÁRIA, FIBROSE, FÍGADO, MORFOMETRIA, VETERINARY MEDICINE, FIBROSIS, LIVER, MORPHOMETRY, PATOLOGIA EXPERIMENTAL, EXPERIMENTAL PATHOLOGY