Quantifying hepatic fibrosis on murine models: how to obtain representative results in a less laborious way

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Data

2019

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Editora

Edições Universitárias Lusófonas

Resumo

In biomedical research, quantification of histological images is often required. Many of the methods used are time-consuming, laborious, originate variable results and are difficult to replicate. This paper is aimed towards finding a more objective, reproducible and easy to perform method to obtain representative results in murine models of hepatic fibrosis using ImageJ software. To do so on a liver fibrosis model, the percentages of fibrotic lesion obtained in an entire section and in several different magnifications were compared. No statistically significant differences were found (p> 0.05), but the correlation was stronger between the results obtained in the entire section photograph and the two photographs at 40x (r = 0.963). Using ImageJ facilitated the definition of a methodology that originated representative results on a liver section and at the same time allowed objective measurement in a reproducible and less laborious way.
In biomedical research, quantification of histological images is often required. Many of the methods used are time-consuming, laborious, originate variable results and are difficult to replicate. This paper is aimed towards finding a more objective, reproducible and easy to perform method to obtain representative results in murine models of hepatic fibrosis using ImageJ software. To do so on a liver fibrosis model, the percentages of fibrotic lesion obtained in an entire section and in several different magnifications were compared. No statistically significant differences were found (p> 0.05), but the correlation was stronger between the results obtained in the entire section photograph and the two photographs at 40x (r = 0.963).

Descrição

Revista Lusófona de Ciência e Medicina Veterinária

Palavras-chave

IMAGEJ, FÍGADO, MORFOMETRIA, VETERINÁRIA, MEDICINA VETERINÁRIA, FIBROSE, PATOLOGIA EXPERIMENTAL, VETERINARY MEDICINE, FIBROSIS, LIVER, MORPHOMETRY, EXPERIMENTAL PATHOLOGY

Citação

Catarino , J C M , Viegas , I , Rodrigues , J , Pinto , M & Faísca , P 2019 , ' Quantifying hepatic fibrosis on murine models: how to obtain representative results in a less laborious way ' , Default journal . https://doi.org/10.60543/rlcmv.v9i0.6658

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