Caracterização clínica e laboratorial de gatos considerados suspeitos de mycoplasma haemofelis
Miniatura indisponível
Data
2015
Autores
Título da revista
ISSN da revista
Título do Volume
Editora
Resumo
A Mycoplasma haemofelis é uma bactéria gram-negativa que infecta gatos domésticos ou selvagens. Os animais que recuperam da infecção aguda permanecem cronicamente infectados durante meses, anos ou até mesmo durante toda a sua vida, não apresentando quaisquer sinais clínicos. Neves (2013) testou em Portugal 58 gatos para Mhf (sem sinais clínicos compatíveis com infecção por esta bactéria) e verificou uma prevalência de 20.7%, tendo sido utilizada a técnica de PCR nas amostras sanguíneas.
Este estudo retrospectivo teve como objectivo geral investigar a presença de diferenças entre o perfil clínico, hematológico e bioquímico e possíveis factores de risco em animais clinicamente suspeitos de infecção por Mycoplasma haemofelis, que foram consultados no Hospital Veterinário CASVET, Parede. A pesquisa do agente foi realizada pela técnica de PCR em tempo real (RT-PCR) em amostras sanguíneas.
Verificou-se presença estatisticamente significativa de trombocitopenia em animais positivos para Mycoplasma haemofelis [Mhf (+)]. Por outro lado, estes animais cujo diagnóstico foi positivo apresentaram normoproteinémia estatisticamente significativa em relação aos animais Mhf (-) que apresentavam hiperproteinémia. Não se encontraram quaisquer outras diferenças para os restantes parâmetros hematológicos, bioquímicos e de caracterização dos animais não existindo também relação estatística entre a presença de anemia nos animais infectados e o seu estado retroviral.
Este trabalho é muito sugestivo da elevada prevalência da infecção por Mhf na clínica em estudo, sendo muitos animais portadores assintomáticos. Deste modo, a pesquisa do Mhf em contexto clínico deverá ter em conta pelo menos mais do que um dos sinais clínicos, uma vez que a maioria dos mesmos não são patognomónicos. Outros testes de diagnóstico antes da realização do teste de PCR são imprescindíveis para uma melhor detecção de animais com a fase aguda da doença.
Mycoplasma haemofelis is a gram-negative bacteria which infects both domestic and wild cats. Animals that recover from acute infection remain chronically infected for months, years or even throughout his life, showing no clinical signs. Neves, 2013, tested in Portugal 58 cats for Mhf (without clinical signs consistent with infection by this bacteria) and found a prevalence of 20.7%, having been used PCR in blood samples. This retrospective study had as a general objective investigate the presence of differences between the clinical, hematological and biochemical profile and possible risk factors for animals clinically suspected of infection with Mycoplasma haemofelis, who were consulted at CASVET, a veterinary hospital in Parede, Portugal. Search agent was performed by real time PCR (RT- PCR) in blood samples. There was a statistically significant presence of thrombocytopenia in animals positive for Mycoplasma haemofelis [Mhf (+)]. Moreover, this animals whose diagnosis was positive showed a statistically significant normproteinaemia compared to [Mhf (-)] that had hyperproteinemia. They did not match any other differences for the remaining hematological and biochemical parameters and characterization of the animals. There was also no statistical relationship between the presence of anemia and the retroviral state. This work is very suggestive of the high prevalence of infection by Mhf in CASVET, with various animals being asymptomatic carriers. Mhf research in the clinical setting should take into account at least more than one clinical sign, once most of them are not pathognomonic. Other diagnostic tests prior to the PCR testing are vital for improved detection of animals in the acute phase of the disease.
Mycoplasma haemofelis is a gram-negative bacteria which infects both domestic and wild cats. Animals that recover from acute infection remain chronically infected for months, years or even throughout his life, showing no clinical signs. Neves, 2013, tested in Portugal 58 cats for Mhf (without clinical signs consistent with infection by this bacteria) and found a prevalence of 20.7%, having been used PCR in blood samples. This retrospective study had as a general objective investigate the presence of differences between the clinical, hematological and biochemical profile and possible risk factors for animals clinically suspected of infection with Mycoplasma haemofelis, who were consulted at CASVET, a veterinary hospital in Parede, Portugal. Search agent was performed by real time PCR (RT- PCR) in blood samples. There was a statistically significant presence of thrombocytopenia in animals positive for Mycoplasma haemofelis [Mhf (+)]. Moreover, this animals whose diagnosis was positive showed a statistically significant normproteinaemia compared to [Mhf (-)] that had hyperproteinemia. They did not match any other differences for the remaining hematological and biochemical parameters and characterization of the animals. There was also no statistical relationship between the presence of anemia and the retroviral state. This work is very suggestive of the high prevalence of infection by Mhf in CASVET, with various animals being asymptomatic carriers. Mhf research in the clinical setting should take into account at least more than one clinical sign, once most of them are not pathognomonic. Other diagnostic tests prior to the PCR testing are vital for improved detection of animals in the acute phase of the disease.
Descrição
Orientação : Joana de
Oliveira ; co-orientação : Odete Almeida
Palavras-chave
MESTRADO INTEGRADO EM MEDICINA VETERINÁRIA, VETERINÁRIA, GATOS, INFEÇÕES BACTERIANAS, BIOQUÍMICA VETERINÁRIA, HEMATOLOGIA VETERINÁRIA, SINTOMATOLOGIA, CATS, BACTERIAL INFECTIONS, VETERINARY BIOCHEMISTRY, VETERINARY HEMATOLOGY, SYMPTOMATOLOGY, FELÍDEOS, FELIDS, MEDICINA VETERINÁRIA, VETERINARY MEDICINE