Avaliação dos níveis séricos de proteína c reativa em cães com doença periodontal
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2016
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A doença periodontal é um processo inflamatório do periodonto bastante prevalente
em cães e associado a um estadio crónico de inflamação sistémica, podendo este ser
alterado após tratamento adequado. A proteína C reativa (CRP) é uma proteína de fase
aguda utilizada como diagnóstico de doença sistémica.
O presente estudo teve como objetivo determinar os efeitos sistémicos da doença
periodontal através do doseamento da CRP sérica e avaliar o impacto do tratamento
periodontal em cães doentes, comparando os níveis de CRP com cães saudáveis, sem
doença periodontal (grupo controlo).
A população estudada incluiu os animais atendidos nos serviços de Medicina
Dentária da Clínica Veterinária AniAid e da Faculdade de Medicina Veterinária da
Universidade Lusófona, durante o período compreendido entre 1 de setembro e 31 de
dezembro de 2015. Estes animais foram submetidos a exame físico e estomatológicodentário,
análises hematológicas e radiografia intra-oral. Para o estudo foram selecionados
39 cães divididos entre dois grupos: (i) grupo controlo constituído por 28 animais com idade
inferior a 2 anos e sem qualquer doença inflamatória, incluindo doença periodontal e (ii)
grupo com doença constituído por 11 animais com mais de 2 anos de idade e com doença
periodontal. O doseamento da CRP sérica foi realizado com recurso ao kit Canine-CRP
(Randox, República da Irlanda). No grupo controlo, obteve-se uma amostra de sangue e no
grupo com doença, duas amostras (uma antes e outra 30 dias após o tratamento
periodontal).
Diferenças significativas (p=0,001) foram encontradas entre o grupo controlo (2,947 ±
1,428 mg/L) e pré-tratamento (13,824 ± 17,273 mg/L), no entanto, entre o grupo controlo e
pós-tratamento, não existiram diferenças significativas (3,376 ± 1,235 mg/L). Os valores de
CRP entre o grupo controlo e pós-tratamento foram significativamente mais baixos que os
obtidos no grupo pré-tratamento.
O doseamento de CRP em cães com doença periodontal foi avaliado em estudos
anteriores, incluindo pré e pós-tratamento. Contudo, os valores de CRP reportados na
presença de doença periodontal nunca foram comparados com um grupo de controlo. Este
estudo permitiu caracterizar a associação entre a doença periodontal e o estadio
inflamatório sistémico. A análise utilizada neste estudo foi de elevada especificidade para o
cão, permitindo uma determinação mais precisa dos valores séricos, sendo que, de acordo
com o conhecimento dos autores, não foi utilizada anteriormente em estudos clínicos de
doença periodontal em cães.
Periodontal disease is an periodontium inflammatory process highly prevalent in dogs and associated with a chronic systemic inflammatory status. Appropriate treatment for PD can cause a shift of this status. C reactive protein (CRP) is an acute phase protein used as a diagnostic indicator of systemic disease. The present study aimed to determine the systemic effects of periodontal disease through the measurement of serum CRP and to evaluate the impact of periodontal treatment in diseased dogs comparing CRP levels with healthy dogs without periodontal disease (control group). The study population includes dogs selected during the period between September 1st and December 31th of 2015, from two veterinary centers in Lisbon. These dogs were subjected to a detailed physical examination and dental charting, haematological analysis and intra-oral radiography. For the study were selected 39 dogs divided into two groups: (i) control group comprised 28 animals under 2 years old without any inflammatory condition, including periodontal disease and (ii) the disease group comprised 11 animals over 2 years old diafnosed with periodontal disease. Serum CRP measurement was performed using the kit Canine-CRP (Randox, Ireland). In the control group there was obtained a blood sample and in the disease group, two samples (one before and another 30 days after periodontal treatment). Significant differences (p=0.001) were found between the control (2.947 ± 1.428 mg/L) and pre-treatment diseased group (13.824 ± 17.273 mg/L) but not between the control and post-treatment diseased group (3.376 ± 1.235 mg/L). CRP values between control and post-treatment disease group were significantly lower than those obtained in pre-treatment diseased group. CRP measurement has been previously assessed in the dog with periodontal disease, including pre and post periodontal treatment. However, the CRP values reported were always in the presence of periodontal disease and never have been compared with a control group. This study allowed a more accurate establishment of the association between periodontal disease and systemic inflammatory status by comparing the values with a normal control group. Additionally, the specific analytical sort used in this study was highly specific for this species, allowing a more accurate determination of all values, not being, as for the author’s knowledge, previously used in clinical studies of periodontal disease in dogs.
Periodontal disease is an periodontium inflammatory process highly prevalent in dogs and associated with a chronic systemic inflammatory status. Appropriate treatment for PD can cause a shift of this status. C reactive protein (CRP) is an acute phase protein used as a diagnostic indicator of systemic disease. The present study aimed to determine the systemic effects of periodontal disease through the measurement of serum CRP and to evaluate the impact of periodontal treatment in diseased dogs comparing CRP levels with healthy dogs without periodontal disease (control group). The study population includes dogs selected during the period between September 1st and December 31th of 2015, from two veterinary centers in Lisbon. These dogs were subjected to a detailed physical examination and dental charting, haematological analysis and intra-oral radiography. For the study were selected 39 dogs divided into two groups: (i) control group comprised 28 animals under 2 years old without any inflammatory condition, including periodontal disease and (ii) the disease group comprised 11 animals over 2 years old diafnosed with periodontal disease. Serum CRP measurement was performed using the kit Canine-CRP (Randox, Ireland). In the control group there was obtained a blood sample and in the disease group, two samples (one before and another 30 days after periodontal treatment). Significant differences (p=0.001) were found between the control (2.947 ± 1.428 mg/L) and pre-treatment diseased group (13.824 ± 17.273 mg/L) but not between the control and post-treatment diseased group (3.376 ± 1.235 mg/L). CRP values between control and post-treatment disease group were significantly lower than those obtained in pre-treatment diseased group. CRP measurement has been previously assessed in the dog with periodontal disease, including pre and post periodontal treatment. However, the CRP values reported were always in the presence of periodontal disease and never have been compared with a control group. This study allowed a more accurate establishment of the association between periodontal disease and systemic inflammatory status by comparing the values with a normal control group. Additionally, the specific analytical sort used in this study was highly specific for this species, allowing a more accurate determination of all values, not being, as for the author’s knowledge, previously used in clinical studies of periodontal disease in dogs.
Descrição
Orientação : João Filipe Requicha ; co-orientação : Lisa Mestrinho
Palavras-chave
MESTRADO INTEGRADO EM MEDICINA VETERINÁRIA, VETERINÁRIA, CÃES, DOENÇAS PERIODONTAIS, PROTEÍNAS, INFLAMAÇÕES, DOGS, PERIODONTAL DISEASES, PROTEINS, INFLAMMATION, CANÍDEOS, CANIDS, MEDICINA VETERINÁRIA, VETERINARY MEDICINE