Linfoma canino: estudo de 307 casos com imunofenotipagem em lâminas citológicas previamente coradas
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2021
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O linfoma é uma das neoplasias caninas mais frequentes, podendo ser facilmente
diagnosticado por citologia. A determinação do imunofenótipo é importante para o tratamento
e prognóstico, podendo realizar-se através de imunocitoquímica. O uso de lâminas citológicas
previamente coradas é ainda pouco instituído mas amplamente desejável, pois permite uma
prévia validação da qualidade da amostra.
Este trabalho teve como objetivos: determinar o imunofenótipo de 307 casos de
linfoma canino através de imunocitoquímica em lâminas citológicas previamente coradas,
realizar uma análise descritiva da amostra e determinar a relação entre o imunofenótipo e as
variáveis raça, porte, idade e sexo.
Recolheram-se dados referentes aos casos submetidos ao laboratório CEDIVET no
período de Abril/2020 a Março/2021 para imunofenotipagem de linfoma em lâminas citológicas
previamente coradas (n=384). Foram incluídos no estudo amostras de linfonodos, baço, fígado
e massas extra-cavitárias com diagnóstico citológico de linfoma. Foram excluídos os casos
referentes à espécie felina ou cuja amostra fosse esfregaço sanguíneo (n=77). Realizou-se
análise estatística descritiva e estatística inferencial.
O imunofenótipo foi determinado em 92,8% dos casos, com 84,2% linfomas B e
15,8% linfomas T. Observou-se um predomínio de cães sem raça definida/cruzados,
maioritariamente machos, de porte grande e com uma média de idade de 8,6 anos. Verificou-se
uma relação estatisticamente significativa entre o imunofenótipo T e a raça Boxer.
A imunocitoquímica possibilitou a imunofenotipagem da maioria dos casos.
Verificou-se a existência de relação entre o imunofenótipo e raça. Foi, ainda, o primeiro estudo
em Portugal a referir a utilização de lâminas citológicas previamente coradas para análise
imunocitoquímica, tendo uma casuística notoriamente superior aos poucos previamente
reportados na literatura mundial.
Lymphoma is one of the most frequent canine neoplasms and can be easily diagnosed by cytology. Classifying the immunophenotype is important for treatment and prognosis, and it can be done through immunocytochemistry. The use of previously stained cytological slides is poorly established but highly desirable, as it allows a previous validation of the quality of the sample. This study aimed to classify 307 cases of canine lymphoma through immunocytochemistry on previously stained cytological slides, perform a descriptive analysis of the sample and evaluate the relationship between the immunophenotype and variables such as breed, size, age, and sex. Data referring to cases submitted to the CEDIVET laboratory between April/2020 and March/2021 for lymphoma immunophenotyping on previously stained cytological slides were collected (n=384). Samples from lymph nodes, spleen, liver and extra-cavitary masses with cytological diagnosis of lymphoma were included in the study. Cases referring to the feline species or whose sample was a blood smear (n=77) were excluded. Descriptive and inferential statistical analysis were performed. Immunophenotype's classification was achieved in 92.8% of the cases, with 84.2% Bcell lymphomas and 15.8% T-cell lymphomas. Most of the dogs were mixed and large breeds, males and with an average age of 8,6 years. There was a statistically significant relationship between the T-cell immunophenotype and the Boxer breed. Immunophenotyping through immunocytochemistry was possibly in most of the cases. There was a relationship between immunophenotype and race. It was the first study in Portugal reporting the use of previously stained slides for immunocytochemical analysis, with a remarkable higher number of cases comparing to the few first reported in the world literature.
Lymphoma is one of the most frequent canine neoplasms and can be easily diagnosed by cytology. Classifying the immunophenotype is important for treatment and prognosis, and it can be done through immunocytochemistry. The use of previously stained cytological slides is poorly established but highly desirable, as it allows a previous validation of the quality of the sample. This study aimed to classify 307 cases of canine lymphoma through immunocytochemistry on previously stained cytological slides, perform a descriptive analysis of the sample and evaluate the relationship between the immunophenotype and variables such as breed, size, age, and sex. Data referring to cases submitted to the CEDIVET laboratory between April/2020 and March/2021 for lymphoma immunophenotyping on previously stained cytological slides were collected (n=384). Samples from lymph nodes, spleen, liver and extra-cavitary masses with cytological diagnosis of lymphoma were included in the study. Cases referring to the feline species or whose sample was a blood smear (n=77) were excluded. Descriptive and inferential statistical analysis were performed. Immunophenotype's classification was achieved in 92.8% of the cases, with 84.2% Bcell lymphomas and 15.8% T-cell lymphomas. Most of the dogs were mixed and large breeds, males and with an average age of 8,6 years. There was a statistically significant relationship between the T-cell immunophenotype and the Boxer breed. Immunophenotyping through immunocytochemistry was possibly in most of the cases. There was a relationship between immunophenotype and race. It was the first study in Portugal reporting the use of previously stained slides for immunocytochemical analysis, with a remarkable higher number of cases comparing to the few first reported in the world literature.
Descrição
Orientação: Nazaré Pinto da Cunha ; coorientação: Joana Fonseca
Palavras-chave
MESTRADO INTEGRADO EM MEDICINA VETERINÁRIA, MEDICINA VETERINÁRIA, VETERINÁRIA, CÃES, CANÍDEOS, CASOS CLÍNICOS, LINFOMA, CITOLOGIA, VETERINARY MEDICINE, CASE REPORTS, CANIDS, DOGS, LYMPHOMA, CYTOLOGY, IMUNO-HISTOQUÍMICA, IMMUNOHISTOCHEMISTRY