Terapêutica de células estaminais na osteoartrite grave em cães
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Data
2022
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Resumo
A osteoartrite é uma doença crónica multifatorial resultante de alterações da
homeostase, que condiciona a capacidade funcional do doente. O presente estudo pretende
comprovar a tolerância, a eficácia, a repetibilidade e a segurança do protocolo multimodal de
modalidades de reabilitação funcional e medicina regenerativa por células estaminais nesta
doença.
Oito cães (n=8) foram selecionados no período entre 14 de setembro de 2020 e 15 de
dezembro de 2021 e com média de idades de 9,5 anos e de peso vivo 35,63Kg, com osteoartrite
coxofemoral ou femuro-tibio-patelar e com dor e claudicação graves.
Todos foram avaliados em consulta de reabilitação e regeneração, incluindo a
realização do exame de reabilitação funcional, avaliação de graus de dor e claudicação, medição
de amplitude articular e massa muscular. Foram administradas, intra-articularmente, células
estaminais alogénicas da membrana sinovial com aplicação de ondas choque e laserterapia
classe IV. A monitorização realizou-se no momento da admissão, ao fim do 7º e 14º dia da
administração das células estaminais, com consultas de seguimento após 30, 90, 180, 270 e 365
dias.
No estudo constatou-se uma melhoria em todos os parâmetros avaliados, tendo-se
observado que, em F5, n=4 apresentaram dor e claudicação ligeiras e n=8 melhoraram a
amplitude articular e massa muscular, obtendo, por isso, qualidade de vida até, pelo menos 365
dias.
A aplicação do presente protocolo multimodal sugere redução de ambas as escalas,
podendo-se concluir que este mantém a qualidade de vida a longo termo. Contudo, sendo a
amostra reduzida, serão necessários mais estudos para avaliar a potencialidade do protocolo
apresentado.
Osteoarthritis is a chronic multifactorial disease resulting from changes in homeostasis, leading to the functional disability. The present study intends to prove the tolerance, the efficacy, the repeatability and the safety of the multimodal protocol of functional rehabilitation and stem cell regenerative medicine modalities in this disease. Eight dogs (n=8) were selected in the period between September 14, 2020 and December 15, 2021 and with mean age of 9.5 years-old and body weight of 35.63 kg, with coxofemoral or femoro-tibio-patellar osteoarthritis and severe pain and claudication. All were evaluated in animal rehabilitation and regeneration appointments composed of functional rehabilitation examination, assessment of degrees of pain and lameness, measurement of joint amplitude and muscle mass. Allogeneic stem cells from the synovial membrane were administered intra-articularly with the administration of shock waves and class IV lasertherapy. Monitoring was completed at admission, after the 7th and 14th day after stem cell administration, and follow-ups after 30, 90, 180, 270 and 365 days. In the study there was an improvement in all parameters evaluated, and it was observed that, in F5, n=4 had mild pain and claudication and n=8 had improved joint amplitude and muscle mass, thus improving their quality of life up to, at least, 365 days. The present multimodal protocol suggests a reduction in pain and claudication scales and it can be concluded that quality of life can be maintained in the long term. However, due to the reduced number of participants more studies should be completed to further evaluate the protocol presented.
Osteoarthritis is a chronic multifactorial disease resulting from changes in homeostasis, leading to the functional disability. The present study intends to prove the tolerance, the efficacy, the repeatability and the safety of the multimodal protocol of functional rehabilitation and stem cell regenerative medicine modalities in this disease. Eight dogs (n=8) were selected in the period between September 14, 2020 and December 15, 2021 and with mean age of 9.5 years-old and body weight of 35.63 kg, with coxofemoral or femoro-tibio-patellar osteoarthritis and severe pain and claudication. All were evaluated in animal rehabilitation and regeneration appointments composed of functional rehabilitation examination, assessment of degrees of pain and lameness, measurement of joint amplitude and muscle mass. Allogeneic stem cells from the synovial membrane were administered intra-articularly with the administration of shock waves and class IV lasertherapy. Monitoring was completed at admission, after the 7th and 14th day after stem cell administration, and follow-ups after 30, 90, 180, 270 and 365 days. In the study there was an improvement in all parameters evaluated, and it was observed that, in F5, n=4 had mild pain and claudication and n=8 had improved joint amplitude and muscle mass, thus improving their quality of life up to, at least, 365 days. The present multimodal protocol suggests a reduction in pain and claudication scales and it can be concluded that quality of life can be maintained in the long term. However, due to the reduced number of participants more studies should be completed to further evaluate the protocol presented.
Descrição
Orientação: Rute Teixeira ; coorientação: Ângela Martins
Palavras-chave
MESTRADO INTEGRADO EM MEDICINA VETERINÁRIA, MEDICINA VETERINÁRIA, OSTEOARTRITE, CÃES, CÉLULAS ESTAMINAIS, CÉLULAS MESENQUIMATOSAS, DOR, REABILITAÇÃO, VETERINARY MEDICINE, OSTEOARTHRITIS, DOGS, STEM CELLS, MESENCHYMAL STEM CELLS, PAIN, REHABILITATION